GERMINATION, GROWTH AND NUTRITIONAL VALUE OF CORN FODDER APPLIED WITH KUSUM TREE (SCHLEICHERA OLEOSA) LIQUID SMOKE
DOI:
https://doi.org/10.22437/jiituj.v9i4.37876Keywords:
Corn Fodder, Germination, Growth, Liquid Smoke, Nutritional ValueAbstract
Fodder is a high-yielding, space-efficient animal feed that can be harvested in a short period, making it crucial for sustainable livestock nutrition. However, improving both the growth and nutritional content of fodder remains a challenge. This study aimed to investigate the effects of liquid smoke application on the germination, growth, and nutritional quality of corn fodder. A Completely Randomized Design (CRD) was used, consisting of five treatments: seeds soaked in 0% (control, P0), 10% (P1), 20% (P2), 30% (P3), and 40% (P4) liquid smoke concentrations for 1 hour. The results showed that liquid smoke treatment did not significantly influence germination parameters, including Final Germination Percentage (FGP), Mean Germination Time (MGT), and Mean Germination Rate (MGR). However, significant effects were observed on growth indicators such as the number of leaves per sprout and dry weight. Furthermore, liquid smoke notably enhanced the crude protein and crude lipid content of the corn fodder, though it did not alter crude fiber levels.This study presents a novel approach to improving fodder quality using plant-derived smoke compounds, offering a simple, low-cost pre-treatment method for enhancing the nutritional profile of corn fodder. The findings imply that liquid smoke can serve as a natural biostimulant, potentially replacing or supplementing synthetic feed additives. Future research should explore the use of smoke water or direct plant-derived smoke under aerobic conditions to optimize germination and further boost fodder quality. These insights contribute to the development of more efficient and sustainable animal feed strategies.
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References
Ahmed, A. A., Aminu, M. S., & Sadauki, S. U. (2023). Seed germination, emergence and seedling vigor of okra (Abelmoschus esculentus L. Moench) as affected by Okra Yellow Vein Mosaic Virus Disease. Nigerian Journal of Horticultural Science, 27(1), 75-83.
Al-Ansari, F., & Ksiksi, T. (2016). A quantitative assessment of germination parameters: The case of and. The Open Ecology Journal, 9, 13–21. https://doi.org/10.2174/1874213001609010013.
Alvi, A. F., Sehar, Z., Fatma, M., Masood, A., & Khan, N. A. (2022). Strigolactone: An emerging growth regulator for developing resilience in plants. Plants, 11(19). https://doi.org/10.3390/plants11192604.
Antala, M. (2022). Chapter 6—Physiological roles of karrikins in plants under abiotic stress conditions. In T. Aftab & M. Naeem (Eds.), Emerging Plant Growth Regulators in Agriculture (pp. 193–204). Academic Press. https://doi.org/10.1016/B978-0-323-91005-7.00016-3.
Antala, M., Sytar, O., Rastogi, A., & Brestic, M. (2020). Potential of karrikins as novel plant growth regulators in agriculture. Plants, 9(1). https://doi.org/10.3390/plants9010043.
AOAC. (1995). Official Methods of Analysis (16th ed.). Association of Official Analytical Chemists.
Armstrong, J., & Heins, B. (2021). Grazing and pasture management for cattle. https://extension.umn.edu/pasture-based-dairy/grazing-and-pasture-management-cattle
Balestrini, R., Chitarra, W., Ghirardo, A., Nardini, A., & Nerva, L. (2022). A stressful life: How plants cope with multiple biotic and abiotic adverse factors. Plant Stress, 5. https://doi.org/10.1016/j.stress.2022.100095.
Bonham, C. D. (2013). Measurements for Terrestrial Vegetation. John Wiley & Sons.
Çatav, S., Küçükakyüz, K., Tavşanoğlu, Ç., & Pausas, J. (2018). Effect of fire-derived chemicals on germination and seedling growth in Mediterranean plant species. Basic and Applied Ecology, 30. https://doi.org/10.1016/j.baae.2018.05.005.
Chaudhary, D., Jat, S., Kumar, R., Kumar, A., & Kumar, B. (2014). Fodder quality of maize: It’s preservation. In Maize: Nutrition Dynamics and Novel Uses (pp. 153–160). https://doi.org/10.1007/978-81-322-1623-0_13.
Cherian, G. (2019). A Guide to the Principles of Animal Nutrition. Oregon State University. https://open.oregonstate.education/animalnutrition/
Daneshvar, A., Tigabu, M., Karimidoost, A., & Odén, P. C. (2017). Flotation techniques to improve viability of Juniperus polycarpos seed lots. Journal of Forestry Research, 28(2), 231–239. https://doi.org/10.1007/s11676-016-0306-2.
Davoudpour, Y., Schmidt, M., Calabrese, F., Richnow, H. H., & Musat, N. (2020). High resolution microscopy to evaluate the efficiency of surface sterilization of Zea Mays seeds. PLOS ONE, 15(11), e0242247. https://doi.org/10.1371/journal.pone.0242247.
Dubois, M. (2022). KUFfed by drought: A KARRIKIN-upregulated F-box protein compromises plant growth and survival under drought. Plant Physiology, 190(4), 2087–2089. https://doi.org/10.1093/plphys/kiac413.
Erdaw, M. M. (2023). Fundamental factors reducing feed quality, efficiency, and carrying-over impacts: a review. Turkish Journal of Agriculture-Food Science and Technology, 11(8), 1422-1431. https://doi.org/10.24925/turjaf.v11i8.1422-1431.6100.
Farooq, M. A., Ma, W., Shen, S., & Gu, A. (2022). Underlying biochemical and molecular mechanisms for seed germination. International Journal of Molecular Sciences, 23(15), 8502. https://doi.org/10.3390/ijms23158502.
Feng, Q., & Lin, Y. (2017). Integrated processes of anaerobic digestion and pyrolysis for higher bioenergy recovery from lignocellulosic biomass: A brief review. Renewable and Sustainable Energy Reviews, 77, 1272–1287. https://doi.org/10.1016/j.rser.2017.03.022.
Fenibo, E. O., & Matambo, T. (2025). Biopesticides for sustainable agriculture: feasible options for adopting cost-effective strategies. Frontiers in Sustainable Food Systems, 9, 1657000. https://doi.org/10.3389/fsufs.2025.1657000.
Feyissa, F., Kebede, G., Geleti, D., Assefa, G., & Mengistu, A. (2025). Improved forage crops research and development in Ethiopia: major achievements, challenges and the way forward. OMO International Journal of Sciences, 8(1), 14-42. https://doi.org/10.59122/135BE51.
Flematti, G., Dixon, K., & Smith, S. (2015). What are karrikins and how were they ‘discovered’ by plants? BMC Biology, 13. https://doi.org/10.1186/s12915-015-0219-0.
Flematti, G. R., Waters, M. T., Scaffidi, A., Merritt, D. J., Ghisalberti, E. L., Dixon, K. W., & Smith, S. M. (2013). Karrikin and cyanohydrin smoke signals provide clues to new endogenous plant signaling compounds. Molecular Plant, 6(1), 29–37. https://doi.org/10.1093/mp/sss132.
Giancaspro, A., Giove, S. L., Zacheo, S. A., Blanco, A., & Gadaleta, A. (2019). Genetic variation for protein content and yield-related traits in a durum population derived from an inter-specific cross between hexaploid and tetraploid wheat cultivars. Frontiers in Plant Science, 10, 1509. https://doi.org/10.3389/fpls.2019.01509.
Gomez, K. A., & Gomez, A. A. (2010). Statistical Procedures for Agricultural Research. Wiley India Pvt. Ltd.
Gong, Z. (2021). Plant abiotic stress: New insights into the factors that activate and modulate plant responses. Journal of Integrative Plant Biology, 63(3), 429. https://doi.org/10.1111/jipb.13079.
Hasanah, U., Azis, P., Jayati, R., Astuti, W., Taskirah, A., Liana, A., Rusmidin, Nopiyanti, N., Lutfi, Veryani, A., Samsi, A., Vertygo, S., Al-Banna, M., & Sulastri, N. (2021). Anatomi dan Fisiologi Tumbuhan [Plant Anatomy and Physiology]. Media Sains Indonesia.
Herbstritt, S., Richard, T. L., Lence, S. H., Wu, H., O’Brien, P. L., Emmett, B. D., Kaspar, T. C., Karlen, D. L., Kohler, K., & Malone, R. W. (2022). Rye as an energy cover crop: Management, forage quality, and revenue opportunities for feed and bioenergy. Agriculture, 12(10), 1691. https://doi.org/10.3390/agriculture12101691.
Herlina, N., & Prasetyorini, A. (2020). Pengaruh perubahan iklim pada musim tanam dan produktivitas jagung (Zea mays L.) di Kabupaten Malang [The effect of climate change on the planting season and productivity of corn (Zea mays L.) in Malang Regency]. Jurnal Ilmu Pertanian Indonesia, 25(1), 118-128. https://doi.org/10.18343/jipi.25.1.118.
Hikmah, A., Luthfianto, D., Silitonga, M., Vertygo, S., Rita, R., Gultom, E., Ulfah, M., & Tika, I. (2022). Buku Ajar Biokimia Teori dan Aplikasi [Biochemistry Textbook: Theory and Applications]. CV. Feniks Muda Sejahtera.
Huang, Y., Wang, H., Zhu, Y., Huang, X., Li, S., Wu, X., Zhao, Y., Bao, Z., Qin, L., Jin, Y., Cui, Y., Ma, G., Xiao, Q., Wang, Q., Wang, J., Yang, X., Liu, H., Lu, X., Larkins, B. A., … Wu, Y. (2022). THP9 enhances seed protein content and nitrogen-use efficiency in maize. Nature, 612(7939). https://doi.org/10.1038/s41586-022-05441-2.
Kandalam, G., & Samireddypalle, A. (2015). Impact of climate change on forage availability for livestock. Climate Change Impact on Livestock: Adaptation and Mitigation, 97–112. https://doi.org/10.1007/978-81-322-2265-1_7.
Kırkpınar, F., Açıkgöz, Z., Kırkpınar, F., & Açıkgöz, Z. (2018). Feeding. In Animal Husbandry and Nutrition. IntechOpen. https://doi.org/10.5772/intechopen.78618.
Koeslulat, E. E. (2016). Karakteristik energi delapan jenis pohon dari kabupaten kupang sebagai dasar perencanaan pengelolaan energi biomasa [Energy characteristics of eight types of trees from Kupang Regency as a basis for biomass energy management planning]. Skripsi Universitas Gadjah Mada. http://etd.repository.ugm.ac.id/penelitian/detail/104780.
Lee, M. A. (2018). A global comparison of the nutritive values of forage plants grown in contrasting environments. Journal of Plant Research, 131(4), 641–654. https://doi.org/10.1007/s10265-018-1024-y.
Malviya, R., & Gayen, D. (2025). Seed deterioration: unraveling the role of phytohormones on seed germination under aging condition. Journal of Plant Growth Regulation, 44(5), 1886-1902. https://doi.org/10.1007/s00344-024-11560-z.
Mekonnen, K., Bezabih, M., Thorne, P., Gebreyes, M. G., Hammond, J., & Adie, A. (2022). Feed and forage development in mixed crop–livestock systems of the Ethiopian highlands: Africa RISING project research experience. Agronomy Journal, 114(1), 46-62. https://doi.org/10.1002/agj2.20853.
Meng, Y., Varshney, K., Incze, N., Badics, E., Kamran, M., Davies, S. F., Oppermann, L. M. F., Magne, K., Dalmais, M., Bendahmane, A., Sibout, R., Vogel, J., Laudencia-Chingcuanco, D., Bond, C. S., Soós, V., Gutjahr, C., & Waters, M. T. (2022). Karrikin Insensitive regulates leaf development, root system architecture and arbuscular-mycorrhizal symbiosis in Brachypodium distachyon. The Plant Journal, 109(6), 1559–1574. https://doi.org/10.1111/tpj.15651.
Murningsih, T., Yulita, K. S., Bora, C. Y., & Arsa, I. G. B. A. (2015). Respon tanaman jagung varietas lokal NTT umur sangat genjah (Pena Tunu’ Ana’) terhadap cekaman kekeringan [Response of maize landrace NTT with very early maturity (Pena Tunu’ Ana’) to drought stress]. Berita Biologi, 14(1), 64272. https://doi.org/10.14203/beritabiologi.v14i1.1865.
Nautiyal, P. C., Sivasubramaniam, K., & Dadlani, M. (2023). Seed dormancy and regulation of germination. Seed science and technology, 52, 39-66. https://doi.org/10.1007/978-981-19-5888-5_3.
Novianda, N., Fajar, B. A., & Fitria, L. (2020). Penerapan budidaya fodder jagung dengan sistem penyiraman timer digital otomatis sebagai pakan kambing etawa dan domba pada kelompok tani di desa benua raja aceh tamiang [Implementation of corn fodder cultivation with an automatic digital timer watering system as feed for Etawa goats and sheep in farmer groups in Benua Raja Village, Aceh Tamiang]. Global Science Society: Jurnal Ilmiah Pengabdian Kepada Masyarakat, 2(1).
Nugroho, A., & Aisyah, I. (2013). Efektivitas asap cair dari limbah tempurung kelapa sebagai biopestisida benih di gudang penyimpanan [The effectiveness of liquid smoke from coconut shell waste as a seed biopesticide in storage warehouses]. Jurnal Penelitian Hasil Hutan, 31(1). https://doi.org/10.20886/jphh.2013.31.1.1-8.
Obroucheva, N. V. (2021). Germination program in non-dormant seeds: Programming, saving and implementation. Russian Journal of Plant Physiology, 68(6), 1003-1017. https://doi.org/10.1134/S1021443721060145.
Poveda, J., Eugui, D., Abril-Urías, P., & Velasco, P. (2021). Endophytic fungi as direct plant growth promoters for sustainable agricultural production. Symbiosis, 85(1), 1-19. https://doi.org/10.1007/s13199-021-00789-x.
Prihartini, R. (2014). Hydroponic fodder sebagai pakan alternatif untuk memenuhi kekurangan hijauan bagi sapi perah selama musim kemarau [Hydroponic fodder as an alternative feed to meet the shortage of green fodder for dairy cows during the dry season]. http://repository.ipb.ac.id/handle/123456789/71473
Reed, H., Felix, T. L., & Clark, B. (2020). What is Your Corn Fodder Worth? https://extension.psu.edu/what-is-your-corn-fodder-worth
Rika, M., Oktavia, S. W., Fitriani, R., & Javed, M. A. (2023). Seed germination test technology practicum (Standard Development Test). Jurnal Ilmiah Ilmu Terapan Universitas Jambi, 7(2). https://doi.org/10.22437/jiituj.v7i2.29761.
Sepulveda, C., Guzmán, M. A., Li, Q., Villaécija-Aguilar, J. A., Martinez, S. E., Kamran, M., Khosla, A., Liu, W., Gendron, J. M., Gutjahr, C., Waters, M. T., & Nelson, D. C. (2022). Karrikin Up-Regulated F-Box 1 (KUF1) imposes negative feedback regulation of karrikin and KAI2 ligand metabolism in Arabidopsis thaliana. Proceedings of the National Academy of Sciences, 119(11), e2112820119. https://doi.org/10.1073/pnas.2112820119.
Shan, Z., Zhou, S., Shah, A., Arafat, Y., Arif Hussain Rizvi, S., & Shao, H. (2023). Plant allelopathy in response to biotic and abiotic factors. Agronomy, 13(9), 2358. https://doi.org/10.3390/agronomy13092358.
Singh, D. N., Bohra, J. S., Tyagi, V., Singh, T., Banjara, T. R., & Gupta, G. (2022). A review of India’s fodder production status and opportunities. Grass and Forage Science, 77(1), 1-10. https://doi.org/10.1111/gfs.12561.
Sitorus, R. M. (2020). Evaluasi nutrisi fodder jagung sistem hidroponik dengan umur panen yang berbeda sebagai pengganti pakan konsentrat [Nutritional evaluation of corn fodder in hydroponic systems with different harvest ages as a substitute for concentrate feed]. Skripsi. Universitas Pancabudi
Srivastava, A. K., Suresh Kumar, J., & Suprasanna, P. (2021). Seed ‘primeomics’: plants memorize their germination under stress. Biological Reviews, 96(5), 1723-1743. https://doi.org/10.1111/brv.12722.
Soverda, N., Indraswari, E., & Neliyati, N. (2022). Pengaruh aplikasi trichokompos pelepah kelapa sawit terhadap pertumbuhan tanaman timun (Cucumis sativus L) [The effect of trichocompost application from oil palm fronds on the growth of cucumber plants (Cucumis sativus L)]. Jurnal Ilmiah Ilmu Terapan Universitas Jambi, 6(1). https://doi.org/10.22437/jiituj.v6i1.19332.
Tang, B., Vertygo, S., Lema, A., & Swari, W. (2020). Analisis laju perkecambahan kacang tanah (Arachis hypogaea (L.) Merr.) yang diberikan kombinasi perlakuan suhu dan lama perendaman asap cair (Liquid Smoke) [Analysis of the germination rate of peanuts (Arachis hypogaea (L.) Merr.) given a combination of temperature treatment and duration of immersion in liquid smoke]. Jurnal Penelitian Pertanian Terapan, 20, 65. https://doi.org/10.25181/jppt.v20i1.1482.
Tuan, P. A., Sun, M., Nguyen, T.-N., Park, S., & Ayele, B. T. (2019). 1—Molecular mechanisms of seed germination. In H. Feng, B. Nemzer, & J. W. DeVries (Eds.), Sprouted Grains (pp. 1–24). AACC International Press. https://doi.org/10.1016/B978-0-12-811525-1.00001-4.
Tufail, M. S., Krebs, G. L., Quddus, M. S., Southwell, A., Piltz, J. W., Norton, M. R., & Wynn, P. C. (2025). Empowering smallholder farmers by integrating participatory research and establishing village-based forage seed enterprises to enhance on-farm productivity and local seed supply. Seeds, 4(3), 40. https://doi.org/10.3390/seeds4030040.
Uslan, U., & Jannah, N. (2020). Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker. Biodiversitas Journal of Biological Diversity, 21(3). https://doi.org/10.13057/biodiv/d210348.
Van de Velde, E., Van Wilder, L., Schindfessel, C., Vermeir, P., Geelen, D., Steppe, K., ... & Dhooghe, E. (2025). Far-red increases tipburn development at different PPFDs and planting densities in an indoor vertical farm. Scientia Horticulturae, 345, 114142. https://doi.org/10.1016/j.scienta.2025.114142.
Vertygo, S. (2021). Biologi Dasar I: Untuk Teknologi Pakan Ternak [Basic Biology I: For Animal Feed Technology]. Syiah Kuala University Press. https://scholar.google.com/citations?view_op=view_citation&hl=en&user=5vokahcAAAAJ&cstart=20&pagesize=80&citation_for_view=5vokahcAAAAJ:hqOjcs7Dif8C
Vertygo, S., & Belni S. Naimasus, B. N. G. (2022). Respon germinasi dan pertumbuhan jagung putih lokal timor yang diaplikasikan dengan asap cair dari berbagai sumber material tumbuhan [Germination and growth response of local Timor white corn applied with liquid smoke from various plant material sources]. Prosiding Seminar Nasional Penelitian, 5(1). https://ejurnal.politanikoe.ac.id/index.php/psnp/article/view/110
Vertygo, S., Naimasus, B. S., Go’o, B. N., Mate, R. L., & Tang, B. Y. (2022). Aplikasi asap cair berbasis kesambi dengan konsentrasi berbeda terhadap perkecambahan jagung putih lokal timor [Application of kesambi-based liquid smoke with different concentrations on the germination of local Timor white corn]. Partner, 27(2). https://doi.org/10.35726/jp.v27i2.1000.
Wang, L., Waters, M. T., & Smith, S. M. (2018). Karrikin-KAI2 signalling provides Arabidopsis seeds with tolerance to abiotic stress and inhibits germination under conditions unfavourable to seedling establishment. New Phytologist, 219(2), 605–618. https://doi.org/10.1111/nph.15192.
Wattanaklang, B., Abrar, A., & Cherdthong, A. (2016). Nutritional value of fermented maize stover as feed for ruminant. Jurnal Peternakan Sriwijaya, 5(1). https://doi.org/10.33230/JPS.5.1.2016.3919.
Widiastuti, S., Nugraha, N. A. P., Rani, D. M., & Rahayu, T. P. (2022). Evaluation of corn fodder hydroponic nutrient content as a substitute of forgive livestock feed. Jurnal Ilmiah Peternakan Terpadu, 10(1). https://doi.org/10.23960/jipt.v10i1.p28-38
Wu, G. (2017). Principles of Animal Nutrition. CRC Press.
Xu, H., Wang, F., Damari, R. N., Chen, X., & Lin, Z. (2024). Molecular mechanisms underlying the signal perception and transduction during seed germination. Molecular Breeding, 44(4), 27. https://doi.org/10.1007/s11032-024-01465-w.
Zbancă, A., Rusu, T., Panuța, S., & Negritu, G. (2025). Conservation agriculture as a pathway to climate and economic resilience for farmers in the Republic of Moldova. Sustainability, 17(24), 10916. https://doi.org/10.3390/su172410916.
Zhang, Q. P., Wang, J., & Wang, Q. (2021). Effects of abiotic factors on plant diversity and species distribution of alpine meadow plants. Ecological Informatics, 61, 101210. https://doi.org/10.1016/j.ecoinf.2021.101210
Zwanenburg, B., & Blanco-Ania, D. (2018). Strigolactones: New plant hormones in the spotlight. Journal of Experimental Botany, 69(9), 2205–2218. https://doi.org/10.1093/jxb/erx487.
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