Analysis of quercetin content as an anti-inflammatory agent in Akasia leaf extract (Acacia auriculiformis) using the HPLC method

Zahra Frizki Asty; Annissa Delfira; Denok Tri Hardiningsih; Zilzikridini Wijayanti

doi:10.22437/proca.v1i2.50263

Authors

Zahra Frizki Asty Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universitas Jambi
Annissa Delfira Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universitas Jambi
Denok Tri Hardiningsih Department of Phytopharmacy, Faculty of Medicine and Health Sciences, Universitas Jambi
Zilzikridini Wijayanti Department of Public Health Sciences, Faculty of Medicine and Health Sciences, Universitas Jambi

DOI:

https://doi.org/10.22437/proca.v1i2.50263

Keywords:

Quercetin, Acacia auriculiformis, HPLC, Flavonoid, Anti-inflammatory

Abstract

Background: Inflammation is a natural defense response, characterized by five classic symptoms, namely swelling, heat, redness, pain, and impaired organ function. However, uncontrolled inflammation can trigger various chronic diseases, so effective and safe anti-inflammatory agents are needed. One natural compound that has this potential is quercetin, a flavonoid with antioxidant and anti-inflammatory activities through inhibition of cyclooxygenase (COX) and lipoxygenase (LOX) enzymes, as well as modulation of the NF-κB transcription factor. Acacia auriculiformis leaves are known to contain quercetin and other bioactive compounds, so they have the potential to be developed as a source of natural anti-inflammatory agents. Objective: This study aims to analyze the quercetin content in ethanol extract of Acacia leaves using high-performance liquid chromatography (HPLC). Methods: Extraction was performed by maceration using 96% ethanol. Acacia leaf simplicia weighing 300 grams was successfully extracted and produced a thick extract of 30 ml with a characteristic dark green-brown color. Then, it was analyzed using HPLC based on validation parameters of LOD (0.1172 mg/L) and LOQ (0.3906 mg/L). Results: The quercetin content was found to be below the detection limit (<0.1172 mg/L), making quantitative determination unfeasible. Conclusion: The results suggest that quercetin is present in very low concentrations or possibly in a conjugated form. Further phytochemical and GC-MS analyses are required to confirm the presence and activity of other flavonoids contributing to the anti-inflammatory potential of Acacia leaf extract.

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Author Biographies

Zahra Frizki Asty, Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universitas Jambi

Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universitas Jambi

Annissa Delfira, Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universitas Jambi

Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universitas Jambi

Denok Tri Hardiningsih, Department of Phytopharmacy, Faculty of Medicine and Health Sciences, Universitas Jambi

Department of Phytopharmacy, Faculty of Medicine and Health Sciences, Universitas Jambi

Zilzikridini Wijayanti, Department of Public Health Sciences, Faculty of Medicine and Health Sciences, Universitas Jambi

Department of Public Health Sciences, Faculty of Medicine and Health Sciences, Universitas Jambi

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