Antidiabetic potential of Acacia auriculiformis leaf extract based on antioxidant activity (DPPH assay)
DOI:
https://doi.org/10.22437/proca.v1i2.50264Keywords:
Acacia auriculiformis, DPPH assay, antioxidant, IC₅₀, natural antidiabetic agent, ROS (Reactive Oxygen Species)Abstract
Background: Diabetes mellitus is a chronic metabolic disorder associated with oxidative stress, which leads to complications in various organs. Natural antioxidants from plants have gained attention as potential antidiabetic agents due to their ability to neutralize reactive oxygen species (ROS). Objective: This study aimed to evaluate the antioxidant potential of Acacia auriculiformis leaf extract as a preliminary indicator of its antidiabetic activity using the DPPH radical scavenging method. Methods: The antioxidant activity was determined by the in vitro DPPH (2,2-diphenyl-1-picrylhydrazyl) assay using methanolic extracts of A. auriculiformis leaves at concentrations of 6–100 ppm. The percentage of inhibition was measured spectrophotometrically at 492 nm, and IC₅₀ values were calculated using nonlinear regression analysis with GraphPad Prism 10.3.1. Results: The extract showed a concentration-dependent increase in DPPH radical inhibition, with an IC₅₀ value of 70.09 ppm, classifying it as a strong antioxidant (IC₅₀ < 100 ppm). The correlation analysis (r = 0.9972, p < 0.001) confirmed a strong linear relationship between concentration and antioxidant activity. Conclusion: These findings suggest that A. auriculiformis leaf extract exhibits strong antioxidant activity, likely attributed to its phenolic, flavonoid, and tannin constituents, supporting its potential as a natural candidate for antidiabetic therapy development.
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Copyright (c) 2025 Annissa Delfira, Zahra Frizki Asty, Tengku Arief Buana Perkasa, Hafizah, Zilzikridini Wijayanti
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