Metabolite profile of 25% ethanol extract of Areca nut (Areca catechu) which has potential as an anti-inflammatory
DOI:
https://doi.org/10.22437/proca.v1i2.50267Keywords:
Metabolites, Extract, Ethanol 25%, Areca catechuAbstract
Background: The areca nut plant has been preclinically tested for its pharmacological effects. A 25% ethanol extract is one type of extract suspected to have pharmacological potential. Objective: The purpose of this research is to describe the results of secondary metabolite profiles using LC-MS, which hopefully can provide an overview for further development. Methods: Extraction using the maceration method, then phytochemical screening, and preparation with methanol and DCM, then injected as much as 5 µl into UPLC-QToF-MS/MS, then analyzed with Masslynx 4.1 and Chemspider software. Results: The results have shown that the extract contained 12 compounds, several of which have the potential to be anti-inflammatory. Conclusion: Literature studies have several compounds that have the potential to be anti-inflammatory.
Downloads
References
[1] Sun H, Yu W, Li H, Hu X, Wang X. Bioactive Components of Areca Nut: An Overview of Their Positive Impacts Targeting Different Organs. Nutrients 2024;16. https://doi.org/10.3390/nu16050695.
[2] Dangore-Khasbage S, Bhowate RR, Khubchandani M. Chemical Composition of Areca Nut and Its Adverse Effects on Human Health. Cureus 2023. https://doi.org/10.7759/cureus.43739.
[3] Humaryanto H, Rahman A, Quzwain F. Potential of Areca catechu nut extract for osteoarthritis: Study on rat model. Biomolecular and Health Science Journal 2023;6:48. https://doi.org/10.4103/bhsj.bhsj_34_22.
[4] Humaryanto, Sani F., Fairuz. Pengembangan Produk Herbal Ekstrak Etanol 50% dan Etanol 25% Biji Pinang (Areca catechu) sebagai Agen Antiinflamasi Melalui pemberian Secara Topikal pada Kasus Osteoarthritis. 2023.
[5] Manivasagan P, Venkatesan J, Sivakumar K, Kim SK. Pharmaceutically active secondary metabolites of marine actinobacteria. Microbiol Res 2014;169. https://doi.org/10.1016/j.micres.2013.07.014.
[6] Al-Khayri JM, Rashmi R, Toppo V, Chole PB, Banadka A, Sudheer WN, et al. Plant Secondary Metabolites: The Weapons for Biotic Stress Management. Metabolites 2023;13. https://doi.org/10.3390/metabo13060716.
[7] Velarde-Salcedo AJ, De León-Rodríguez A, Calva-Cruz OJ, Balderas-Hernández VE, De Anda Torres S, Barba-de la Rosa AP. Extraction of bioactive compounds from Rubus idaeus waste by maceration and supercritical fluids extraction: the recovery of high added-value compounds. Int J Food Sci Technol 2023;58. https://doi.org/10.1111/ijfs.16687.
[8] Wu K, Ju T, Deng Y, Xi J. Mechanochemical assisted extraction: A novel, efficient, eco-friendly technology. Trends Food Sci Technol 2017;66. https://doi.org/10.1016/j.tifs.2017.06.011.
[9] Tzanova M, Atanasov V, Yaneva Z, Ivanova D, Dinev T. Selectivity of current extraction techniques for flavonoids from plant materials. Processes 2020;8. https://doi.org/10.3390/pr8101222.
[10] Zhang M, Zhao J, Dai X, Li X. Extraction and Analysis of Chemical Compositions of Natural Products and Plants. Separations 2023;10. https://doi.org/10.3390/separations10120598.
[11] Humaryanto, Sani F., Rahman A.O., Yuliawati, Muhaimin, Khatib A. Pengaruh Variasi Konsentrasi Pelarut Etanol Ekstrak Biji Pinang (Areca cathecu) Terhadap Aktivitas Lipoxygenase. Paten Sederhana 2024.
[12] Ćujić N, Šavikin K, Janković T, Pljevljakušić D, Zdunić G, Ibrić S. Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. Food Chem 2016;194. https://doi.org/10.1016/j.foodchem.2015.08.008.
[13] Septiani G, Susanti S, Sucitra F. Effect of Different Extraction Method on Total Flavonoid Contents of Sansevieria trifasciata P. Leaves Extract. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) 2021;7. https://doi.org/10.22487/j24428744.2021.v7.i2.15573.
[14] El Houda Lezoul N, Belkadi M, Habibi F, Guillén F. Extraction processes with several solvents on total bioactive compounds in different organs of three medicinal plants. Molecules 2020;25. https://doi.org/10.3390/molecules25204672.
[15] Michalak M, Pierzak M, Kręcisz B, Suliga E. Bioactive compounds for skin health: A review. Nutrients 2021;13. https://doi.org/10.3390/nu13010203.
[16] Kumar K, Srivastav S, Sharanagat VS. Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review. Ultrason Sonochem 2021;70. https://doi.org/10.1016/j.ultsonch.2020.105325.
[17] Boyce M, Favela KA, Bonzo JA, Chao A, Lizarraga LE, Moody LR, et al. Identifying xenobiotic metabolites with in silico prediction tools and LCMS suspect screening analysis. Frontiers in Toxicology 2023;5. https://doi.org/10.3389/ftox.2023.1051483.
[18] Abdallah RH, Al-Saleem MSM, Abdel-Mageed WM, Al-Attar ASR, Shehata YM, Abdel-Fattah DM, et al. LCMS/MS Phytochemical Profiling, Molecular, Pathological, and Immune-Histochemical Studies on the Anticancer Properties of Annona muricata. Molecules 2023;28. https://doi.org/10.3390/molecules28155744.
[19] Syarpin, Permatasari S, Pujianto DA. Analysis of phytochemical constituents and antioxidant activity from the fractions of Luvunga sarmentosa root extract using LCMS/MS. Biodiversitas 2023;24. https://doi.org/10.13057/biodiv/d240208.
[20] Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget 2018;9. https://doi.org/10.18632/oncotarget.23208.
[21] V. Stankov S. Definition of Inflammation, Causes of Inflammation and Possible Anti-inflammatory Strategies. Open Inflamm J 2012;5:1–9. https://doi.org/10.2174/1875041901205010001.
[22] Pérez-Cano FJ, Castell M. Flavonoids, inflammation and immune system. Nutrients 2016;8. https://doi.org/10.3390/nu8100659.
[23] Sabu V, Peter J, Sasidharan Nair AIB, Krishnan S, Sathyaseelan Suja LP, Helen A, et al. Combinatorial Action of Triterpenoid, Flavonoid, and Alkaloid on Inflammation. Nat Prod Commun 2019;14. https://doi.org/10.1177/1934578X19868877.
[24] Bai R, Yao C, Zhong Z, Ge J, Bai Z, Ye X, et al. Discovery of natural anti-inflammatory alkaloids: Potential leads for the drug discovery for the treatment of inflammation. Eur J Med Chem 2021;213. https://doi.org/10.1016/j.ejmech.2021.113165.
[25] Ti H, Zhuang Z, Yu Q, Wang S. Progress of plant medicine derived extracts and alkaloids on modulating viral infections and inflammation. Drug Des Devel Ther 2021;15. https://doi.org/10.2147/DDDT.S299120.
[26] Sieniawska E. Activities of tannins-From in Vitro studies to clinical trials. Nat Prod Commun 2015;10:1877–84. https://doi.org/10.1177/1934578x1501001118.
[27] Orso G, Solovyev MM, Facchiano S, Tyrikova E, Sateriale D, Kashinskaya E, et al. Chestnut shell tannins: Effects on intestinal inflammation and dysbiosis in Zebrafish. Animals 2021;11. https://doi.org/10.3390/ani11061538.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Humaryanto, Fairuz, Fathnur Sani Kasmadi, Hanina, Tengku Arief Buana Perkasa

This work is licensed under a Creative Commons Attribution 4.0 International License.
Published with license by LPPM Universitas Jambi. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0 International). This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator.







