Cytotoxicity activity of 50% ethanol extract of areca nut seeds on RAW 264.7 macrophage cells
DOI:
https://doi.org/10.22437/proca.v1i2.50313Keywords:
Cytotoxicity; Ethanol 50%; Areca catechu; RAW 264.7 Macrophage Cells.Abstract
Background: Areca nut is a widely cultivated plant, particularly in Jambi province. This presents an opportunity for its development into a herbal product with pharmacological potential. Cytotoxicity testing plays an important role in the in vitro testing process. Objective: This aims to determine the level of 50% ethanol extract of areca nut seeds that is safe for cell survival. Methods: The concentration of 50% ethanol extract of areca nut seeds that is safe in the cytotoxic test was followed by testing on RAW 264.7 cells (macrophages) stimulated by lipopolysaccharide (LPS) to simulate the inflammatory process. The test concentrations used were 31.25, 62.5, 125, and 250 micrograms/mL. Results: The research results have shown that all concentrations of the 50% ethanol extract of areca nut seeds still exhibit non-toxic properties, with cell viability values above 90%. Conclusion: Concentrations of 31.25, 62.5, 125, and 250 micrograms/mL can be continued for further in vitro testing using RAW 264.7 macrophage cells.
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Copyright (c) 2025 Humaryanto, Fairuz, Fathnur Sani Kasmadi, Wilia Novita Eka Rini, Tengku Arif Buana Perkasa
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