Phytochemical screening of alkaloid compounds on microencapsulation of Moringa oleifera leaf extract

Authors

  • Rita Halim Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Raihanah Suzan Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Hanina Departemen Mikrobiologi dan Parasitologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Hasna Dewi Departemen Histologi dan Patologi Anatomi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Fadliyana Ekawaty Departemen Keperawatan Anak, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Hafizah Departemen Fisiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi
  • Denok Tri Hardiningsih Departemen Fitofarmaka, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

DOI:

https://doi.org/10.22437/proca.v1i2.50324

Keywords:

Microencapsulation; moringa extract; functional foods; antioxidants; alkaloids

Abstract

Background: Moringa oleifera leaves are rich in bioactive compounds, including alkaloids. Alkaloid compounds are crucial for their pharmacological potential and role as natural antioxidants in improving food quality and stability. Objective: This study aimed to examine the presence of alkaloid compounds in the microencapsulation of Moringa oleifera leaf extract as a strategy to protect active compounds from degradation and retain their nutritional benefits. Methods: The Moringa oleifera leaf extract was formulated into microencapsulation using the freeze-drying method. Phytochemical screening for alkaloid compounds was performed using the Dragendorff, Bouchardat, and Wagner tests. Results: The phytochemical screening results confirmed the presence (positive) of alkaloid compounds in the microcapsule preparation. This positive result was characterized by the formation of an intense orange-red precipitate (Dragendorff), a reddish-brown precipitate (Bouchardat), and a brown precipitate (Wagner). Conclusion: The microencapsulation of Moringa oleifera leaf extract successfully presented positively detected alkaloid compounds. The retention of alkaloids as antioxidants in these microcapsules provides a strong foundation for their development as supplements or fortification ingredients in food products to enhance nutritional value.

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Author Biographies

Rita Halim, Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Raihanah Suzan, Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Departemen Gizi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Hanina, Departemen Mikrobiologi dan Parasitologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Departemen Mikrobiologi dan Parasitologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Hasna Dewi, Departemen Histologi dan Patologi Anatomi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Departemen Histologi dan Patologi Anatomi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Fadliyana Ekawaty, Departemen Keperawatan Anak, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Departemen Keperawatan Anak, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Hafizah, Departemen Fisiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Departemen Fisiologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Denok Tri Hardiningsih, Departemen Fitofarmaka, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

Departemen Fitofarmaka, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Jambi

References

[1] Ahmad N, et al. Bioactive compounds in Moringa oleifera: a comprehensive review. J Food Biochem. 2023;47(3):e13987. Available from: https://doi.org/10.1111/jfbc.13987

[2] Farooq F, et al. Medicinal properties of Moringa oleifera: An overview of promising healer. J Med Plants Res. 2012;6(27):4368–74. Available from: https://doi.org/10.5897/JMPR12.279

[3] Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Scientific World J. 2013 Feb 2;2013:162750. Available from: https://doi.org/10.1155/2013/162750

[4] Alia F, et al. The potency of Moringa oleifera lam. As natural antioxidant and its health benefits. Pharm Sci Asia. 2022;49(1):1–10. Available from: https://doi.org/10.29037/psa.v49i1.791

[5] Silva F, et al. Stability of bioactive compounds in food processing: a review. Food Chem. 2024;380:132123. Available from: https://doi.org/10.1016/j.foodchem.2023.132123

[6] Lee JH, et al. Microencapsulation of plant extracts for food applications: recent trends and future perspectives. Food Hydrocolloids. 2025;130:107789. Available from: https://doi.org/10.1016/j.foodhyd.2024.107789

[7] Abramović B, et al. Impact of freeze- and spray-drying microencapsulation techniques on β-glucan powder biological activity: A comparative study. Foods. 2022;11(15):2267. Available from: https://doi.org/10.3390/foods11152267

[8] Szumilo M, et al. Preparation and evaluation of surface solid dispersion of Moringa oleifera leaf extract using freeze-drying method. J Res Pharm. 2025;27(6):2330-41. Available from: https://doi.org/10.1016/j.jrp.2024.08.010

[9] Kadir MA, et al. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. Scientific World J. 2017 Mar 13;2017:3149681. Available from: https://doi.org/10.1155/2017/3149681

[10] Ahmad Nejhad M, et al. Integrated in vitro and in silico evaluation of the antimicrobial and cytotoxic potential of Calotropis procera leaf ethanolic extract: From GC-MS profiling to molecular docking and dynamics. Int J Mol Sci. 2025;26(21):10574. Available from: https://doi.org/10.3390/ijms262110574

[11] Zhang Y, et al. Advances in microencapsulation technology for food bioactive compounds: a review. Trends Food Sci Technol. 2024;130:45-60. Available from: https://doi.org/10.1016/j.tifs.2023.10.009

[12] Putri DA, et al. Development of functional tempe enriched with plant extracts: a review. J Food Sci Technol. 2023;60(5):1234-1245. Available from: https://doi.org/10.1007/s13197-023-05763-7

[13] Gopalakrishnan L, et al. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Sci Hum Wellness. 2016;5(2):49-56. Available from: https://doi.org/10.1016/j.fshw.2016.04.001

[14] Hassan L, et al. Alkaloid bioactive from Annona muricata (Soursop) and its pharmacological benefits. Int J Adv Res. 2022;10(12):803–12. Available from: https://doi.org/10.21474/IJAR01/16024

[15] Nugroho R, et al. Stabilitas antioksidan ekstrak etanol daun kelor (Moringa oleifera Lam) terenkapsulasi dalam maltodekstrin dengan metode spray drying. J Ilm Farm. 2022;18(2):83–91. Available from: https://doi.org/10.20885/jif.v18i2.5539

[16] Madiyanti, et al. Potensi antioksidan dari ekstrak daun kelor (Moringa oleifera Lam.) pada berbagai jenis pelarut. J Trop Pharm Chem. 2023;6(2):167–75. Available from: https://doi.org/10.31539/jtpc.v6i2.4633

[17] Hardiningsih DT, Suhesti TS, Novrial D, Arjadi F. Studi effektivitas nanoemulsi purwoceng pada perbaikan fungsi reproduksi tikus putih jantan pasca induksi sleep deprivation: Study of the Effectiveness of Purwoceng Ethanol Extract Nanoemulsion on Improvement of the Reproductive Function of Male White Rats Post Sleep Deprivation Induction. j. tumbuh. obat. Indonesia. 2023;16(1):30-4. https://doi.org/10.31002/jtoi.v16i1.594

[18] Sarubbo LA, et al. Bioactive compound encapsulation: Characteristics, applications in food systems, and implications for human health. Int J Mol Sci. 2024;25(22):12320. Available from: https://doi.org/10.3390/ijms252212320

[19] Wang Y, et al. Enhancing the stability of Ginkgo biloba extract by microencapsulation using spray drying. Food Sci Technol. 2023;43:e45021. Available from: https://doi.org/10.1590/fst.45021

[20] Yusuf R, et al. Aplikasi teknologi enkapsulasi untuk meningkatkan stabilitas bahan bioaktif: Tinjauan. J Teknol Pertan. 2022;23(2):101–12. Available from: https://doi.org/10.21776/ub.jtp.2022.023.02.3

[21] Rasool S, et al. Microencapsulation: A novel approach for functional food development. J Food Sci Technol. 2024;61(1):1–14. https://doi.org/10.1007/s13197-023-05952-4

[22] Prieto C, et al. Microencapsulation technology for food and nutraceutical applications. Food Rev Int. 2022;38(6):638–60. https://doi.org/10.1080/87559129.2021.1895655

[23] Sudaryati NLG, Utomo B, Adnyana IMDM, et al. Phytochemical profiling, dengue antiviral properties, and cytotoxicity of novel Baper tea polyherbal infusion: Insights from in silico and in vitro studies. Salud, Ciencia y Tecnología. 2025;5:1791. https://doi.org/10.56294/saludcyt20251791

[24] Utami T, et al. Optimasi metode skrining fitokimia untuk senyawa alkaloid pada ekstrak daun kemangi (Ocimum basilicum L.). J Sains Farm Klin. 2023;10(3):201–8. Available from: https://doi.org/10.25077/jsfk.10.3.201-208.2023

[25] Kim T, Park S. Dragendorff reagent: A historical perspective and modern applications in natural product chemistry. Chem Pharm Bull. 2025;73(1):1-10. Available from: https://doi.org/10.1248/cpb.c24-00627

[26] Chen F, Liu G. Optimization of freeze-drying parameters for sensitive bioactive compounds. Food Res Int. 2023;164:112100. https://doi.org/10.1016/j.foodres.2022.112100

[27] Brown K, White J. Quantitative analysis of phenolic compounds in food matrices using HPLC. J Chromatogr A. 2023;1700:463870. https://doi.org/10.1016/j.chroma.2023.463870

[28] Garcia L, Rodriguez M. The role of microencapsulation in enhancing the bioavailability of plant extracts. Nutr Rev. 2024;82(3):210-225. https://doi.org/10.1093/nutrit/nuad090

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Published

30-11-2025

How to Cite

Halim, R., Suzan, R., Hanina, Dewi, H., Ekawaty, F., Hafizah, & Hardiningsih, D. T. (2025). Phytochemical screening of alkaloid compounds on microencapsulation of Moringa oleifera leaf extract. Proceedings Academic Universitas Jambi, 1(2), 716–721. https://doi.org/10.22437/proca.v1i2.50324

Issue

Vol. 1 No. 2 (2025): November

Section

RESEARCH DISSEMINATION

License

Copyright (c) 2025 Rita Halim, Raihanah Suzan, Hanina, Hasna Dewi, Fadliyana Ekawaty, Hafizah, Denok Tri Hardiningsih

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Published with license by LPPM Universitas Jambi. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0 International). This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. 

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