Antioxidant activity and secondary metabolite profile of protocorm-like bodies (PLBs) of Dendrobium crumenatum and Dendrobium liniale
DOI:
https://doi.org/10.22437/proca.v1i2.50455Keywords:
Dendrobium; protocorm-like bodies (PLBs); DPPH; antioxidants; secondary metabolites; IC50Abstract
Background: The Dendrobium orchid genus is recognized as a source of secondary metabolites with potential therapeutic applications. Sustainable biomass availability can be addressed through the induction of protocorm-like bodies (PLBs). Objective: This study aims to observe the growth of PLBs, screen secondary metabolites, and compare the antioxidant activity between PLBs extracts and plant extracts. Methods: The growth of PLBs Dendrobium crumenatum and Dendrobium liniale was observed until day 60. Secondary metabolite screening was carried out qualitatively to identify the presence of alkaloids, phenolics, tannins, flavonoids, saponins, steroids, and terpenoids. Antioxidant activity was measured using the DPPH method on PLBs extracts, plant extracts, and ascorbic acid as a positive control, with the determination of the inhibitory concentration 50 (IC50) values. Results: PLB growth was observed optimally on day 60. Phytochemical examination identified the presence of compounds from the alkaloid, phenolic, tannin, flavonoid, saponin, steroid, and terpenoid groups in all samples. Antioxidant test showed that Dendrobium crumenatum PLBs extract had the most vigorous activity among all samples, with an IC50 value of 76.06 ± 0.41 µg/mL (strong category). This value was superior to that of the plant extract of D. crumenatum, at 175.26 ± 0.46 µg/mL. In general, PLB extracts from both species produced significantly more vigorous antioxidant activity than plant extracts. Conclusion: PLB culture is an effective alternative for producing Dendrobium biomass with a higher content of bioactive compounds. D. crumenatum PLB extract has the potential to be developed as a natural antioxidant agent.
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Copyright (c) 2025 Syamsurizal, Diah Tri Utami, Yuliawati, Elisma, Maimum, Beni Hendro Prabowo
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