Method development and validation of Catechin in aqueous medium by using ultraviolet-visible spectrophotometer
DOI:
https://doi.org/10.22437/proca.v1i2.50529Keywords:
catechin; validation; spectrophotometer; aqueousAbstract
Background: Catechin, a flavonoid compound with significant antioxidant activity, requires a reliable analytical method for its quantitative determination in pharmaceutical and research applications. Objective: In this study, a simple and rapid UV-Visible Spectrophotometry method was developed and validated for the determination of catechin dissolved in aquadest for quality control and laboratory applications. Methods: A UV-Visible spectrophotometry method was developed for the determination catechin dissolved in aquadest. The maximum wavelenght (λmax) of catechin was determined by scanning in the range of 200-400 nm. calibration curves were prepared at concentrations ranging from 20-60 ppm. method validation was carried out according to AOAC (Association of Official Analytical Chemists) guidelines, including asessment of linearity, precision, accuracy, limit of detection (LoD) and limit of quantification (LoQ). Results: Catechin showed maximum absorbantion at 275 nm. The Calibration curve exhibited excellent linearity with a correlation coefficient (R2) of 0,99. precision studies showed %RSD values 7,3%, while accuracy testing yielded recovery values between 95,72±2,42 %- 102,05±1,74%. The LoD and LoQ were calculated to be 4,16 ppm and 12,60 ppm, respectively. Conclusion: the developed UV-Vis Spectrophotometry method is simple, precise, and accurate. it is suitable for routine determination of catechin in aqueous samples and can be applied in research as well as quality control of pharmaceutical formulation.
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Copyright (c) 2025 Puspa Dwi Pratiwi, Novia Tri Astuti, Santi Perawati, Nurul Kamilah Sadli, Vina Neldi, Marizki Pondawinata, Dita Avika Musdhaliva, Wahyu Nidia Putri
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