KANDIDAT IMPLAN TULANG DARI LIMBAH TULANG AYAM DAN AMPAS KOPI ARABIKA KERINCI MENGGUNAKAN METODE HEAT TREATMENT
DOI:
https://doi.org/10.22437/jop.v10i3.46264Keywords:
Fraktur, Hidroksiapatit, Tulang Ayam, Heat Treatment, Ampas KopiAbstract
Peningkatan jumlah kasus patah tulang akibat kecelakaan menuntut pengembangan material pengganti tulang yang efektif. Salah satu alternatif yang potensional adalah hidroksiapatit (HAp), karena memiliki sifat biocompatibility yang sangat mirip dengan jaringan tulang manusia. Tulang ayam diketahui mengandung senyawa kalsium karbonat dan kalsium fosfat yang dapat dimanfaatkan sebagai bahan dasar HAp. Penelitian ini bertujuan untuk mengkarakterisasi HAp yang disintesis dari tulang ayam dan dikombinasikan dengan ampas kopi serta PVA sebagai bahan pengikat. Proses sintesis HAp dilakukan melalui kalsinasi tulang ayam pada suhu 700°C selama 3 jam, diikuti dengan proses ekstraksi kafein dari ampas kopi dan pencampuran bersama larutan HAp dan PVA. Spektrum FTIR menunjukkan keberadaan gugus PO₄³⁻, CO₃²⁻, dan OH⁻. Pola difraksi XRD sesuai dengan standar HAp JCPDS 09-0432, yang menegaskan terbentuknya fase HAp dan ukuran kristal pada masing-masing sampel yaitu 30,88 nm, 27,02 nm, 37,64 nm, 29,86 nm dan 21,61 nm. Hasil SEM menunjukkan adanya pori-pori pada permukaan sampel dengan ukuran berkisar antara 29 µm hingga 401 µm setelah penambahan ampas kopi dan ukuran pori dapat mendukung pertumbuhan sel.
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Adhikara, A. G., Maharani, A. P., Puspitasari, A., Nuswantoro, N. F., Juliadmi, D., Maras, M. A. J., Nugroho, D. B., Saksono, B., & Gunawarman. (2024). Bovine hydroxyapatite for bone tissue engineering: Preparation, characterization, challenges, and future perspectives. European Polymer Journal. 214.
Afifah, F., & Cahyaningrum, S. E. (2020). Sintetis dan Karakterisasi Hidroksiapatiti dari Tulang Sapi (Bos taurus) Menggunakan Teknik Kalsinasi. UNESA Journal of Chemistry. 9(3). 189–196.
Afrilia, D., Bahri, S., Jalaluddin, Masrulita, & ZA, N. (2022). Pengaruh Konsentrasi Ekstrak Kopi Sebagai Inhibitor Terhadap Laju Korosi pada Baja. Chemical Engineering Journal Storage. 4(1): 111–120.
Babakhani, A., Jamaleddin, S., & Olad, A. (2024). Fabrication of magnetic nanocomposite scaffolds based on polyvinyl alcohol-chitosan containing hydroxyapatite and clay modified with graphene oxide : Evaluation of their properties for bone tissue engineering applications. Journal of the Mechanical Behavior of Biomedical Materials. 150.
Bai, L., Li, J., Li, G., Zhou, D., Su, J., & Liu, C. (2025). Skeletal Interoception and Prospective Application in Biomaterials for Bone Regeneration. Bone Research. 13(1): 1–14.
Bee, S. L., & Hamid, Z. A. A. (2020). Hydroxyapatite derived from food industry bio-wastes: Syntheses, properties and its potential multifunctional applications. Ceramics International. 46(11). 17149–17175.
Bee, S., Mariatti, M., Ahmad, N., Yahaya, B. H., & Hamid, Z. A. A. (2019). Effect of the calcination temperature on the properties of natural hydroxyapatite derived from chicken bone wastes. Materials Today: Proceedings, 16(1): 1876–1885.
Budiatin, A. S., Samirah, Apriliani Gani, M., Putri Nilamsari, W., & Ardianto, C. (2020). The characterization of bovine bone-derived hydroxyapatite isolated using novel non-hazardous method. Journal of Biomimetics, Biomaterials and Biomedical Engineering.45:49–56.
Cahyanto, A., Kosasi/h, E., Aripin, D., & Hasratiningsih, Z. (2016). Fabrication of Hydroxyapatite from Fish Bones Waste Using Reflux Method. IOP Conf. Series: Materials Science and Engineering, 172(1): 1–5.
Chocholata, P., Kulda, V., Dvorakova, J., Dobra, J. K., & Babuska, V. (2020). Biological evaluation of polyvinyl alcohol hydrogels enriched by hyaluronic acid and hydroxyapatite. International Journal of Molecular Sciences. 21(16): 1–11.
Cui, L., Zhang, J., Zou, J., Yang, X., Guo, H., Tian, H., Zhang, P., Wang, Y., Zhang, N., Zhuang, X., Li, Z., Ding, J., & Chen, X. (2020). Electroactive composite scaffold with locally expressed osteoinductive factor for synergistic bone repair upon electrical stimulation. Biomaterials. 230.
Darabi, N. H., Kalaee, M., Mazinani, S., & Khajavi, R. (2024). GO/AgNW aided sustained release of ciprofloxacin loaded in Starch/PVA nanocomposite mats for wound dressings application. International Journal of Biological Macromolecules. 266.
Dong, Y., Liang, J., Cui, Y., Xu, S., & Zhao, N. (2018). Fabrication of novel bioactive hydroxyapatite-chitosan-silica hybrid scaffolds: Combined the sol-gel method with 3D plotting technique. Carbohydrate Polymers. 197: 183–193.
El-Bahrawy, N. R., Elgharbawy, H., Elmekawy, A., Salem, M., & Morsy, R. (2024). Development ofporoushydroxyapatite/PVA/gelatin/alginate hybrid flexible scaffolds with improved mechanical properties for bone tissue engineering. Materials Chemistry and Physics. 319: 1–9.
Eliaz, N., & Metoki, N. (2017). Calcium phosphate bioceramics: A review of their history, structure, properties, coating technologies and biomedical applications. Materials. 10(4): 1–104.
Ezerskyte-Miseviciene, A., & Kareiva, A. (2019). Everything old is new again: a reinspection of solid-state method for the fabrication of high quality calcium hydroxyapatite bioceramics. Mendeleev Communications. 29(3): 273–275.
Fonseca, S. C. da, Freitas, R. B., Sotiles, A. R., Schemczssen-Graeff, Z., Miranda, I. M. D. A., Biscaia, S. M. P., Wypych, F., Silva Trindade, E. da, Leão, M. P., Zielak, J. C., and Franco, C. R. C. (2025). 3D scaffold of hydroxyapatite/β tricalcium phosphate from mussel shells: Synthesis, characterization and cytotoxicity. Heliyon. 11(1): 1-13.
Gupta, S., Panneer Selvam, S., Ramadoss, R., and Sundar, S. (2025). Alginate-hydroxyapatite scaffolds: A comprehensive characterization study. Journal of Oral Biology and Craniofacial Research. 15(3): 555–562.
Hanura, A. B., Trilaksani, W., & Suptijah, P. (2017). Karakterisasi Nanohidroksiapatit Tulang Tuna Thunnus sp. Sebagai Sediaan Biomaterial. Jurnal Ilmu Dan Teknologi Kelautan Tropis. 9(2): 619–629.
Hartatiek, Yudyanto, Wuriantika, M. I., Utomo, J., Nurhuda, M., Masruroh, & Santjojo, D. J. D. H. (2020). Nanostructure, porosity and tensile strength of PVA/Hydroxyapatite composite nanofiber for bone tissue engineering. Materials Today: Proceedings. 44: 3203–3206.
Hidayat, T., Triyono, J., & Masykur, A. (2018). Karakterisasi dan Profil Biodegradasi Material Biokomposit Bovine Hidroksiapatit (BHA)/Ampas Kopi/Shellac. Mekanika: Majalah Ilmiah Mekanika. 17(1): 26–32.
Hussain, S., & Sabiruddin, K. (2021). Effect of heat treatment on the synthesis of hydroxyapatite from Indian clam seashell by hydrothermal method. Ceramics International. 47(21): 29660–29669.
Hutama, A. S., & Nugroho, A. (2020). Optimasi Pembuatan Scaffold dengan Struktur Pori-Pori Beraturan Menggunakan Metode Response Surface Method. JMPM (Jurnal Material Dan Proses Manufaktur). 4(1): 1–11.
Kalidas, S., & Sumathi, S. (2025). Biomedical application of gelatin-PVA-silk fibre composite reinforced with copper and manganese substituted hydroxyapatite. Journal of Materials Research and Technology. 34: 1850-1864.
Khalil, M. H., & Zawam, S. H. (2025). Comparative study of Peroneus longus tendon autograft versus Hamstring tendon autograft in arthroscopic anterior cruciate ligament reconstruction. International Orthopaedics, 49(6): 1365–1372.
Kumar, B. Y. S., Kumar, G. C. M., Shahapurkar, K., Tirth, V., Algahtani, A., Al-mughanam, T., Alghtani, A. H., & Murthy, H. C. A. (2023). Processing and characterization of egg shell derived nano-hydroxyapatite synthetic bone for Orthopaedic and Arthroscopy implants and substitutes in dentistry. Journal of the Mechanical Behavior of Biomedical Materials. 144.
Kumar, K. C. V., Subha, T. J., Ahila, K. G., Ravindran, B., Chang, S. W., Mahmoud, A. H., Mohammed, O. B., & Rathi, M. A. (2021). Spectral characterization of hydroxyapatite extracted from Black Sumatra and Fighting cock bone samples: A comparative analysis. Saudi Journal of Biological Sciences. 28(1): 840–846.
Li, H., Wu, C., Yu, X., & Zhang, W. (2023). Recent advances of PVA-based hydrogels in cartilage repair application. Journal of Materials Research and Technology. 24: 2279–2298.
Liandi, A. R., Al-wahid, A. A., Siregar, Y. D. I., Wendari, T. P., Cahyana, A. H., & Insani, A. (2024). Green mussel shell-derived hydroxyapatite-CoFe2O4 catalyst: Microwave-assisted synthesis of 2-amino-4H-chromene derivative Agus. Case Studies in Chemical and Environmental Engineering. 10.
Mahdy, H. M., Hendawy, H., Abbas, Y. M., & Duraia, E. shazly M. (2025). Design and characterization of AgVO3-HAP/GO@PCL ceramic-based scaffolds for enhanced wound healing and tissue regeneration. Journal of Materials Science: Materials in Medicine. 36(1).
Mirwan, A. (2013). Keberlakuan Model HB- GFT Sistem n-Heksana-MEK- Air Pada Ekstraksi Cair-Cair Kolom Isian. Konversi. 2(1): 32–39.
Miura, T., Watanabe, A., Miyake, M., Suga, S., Miyoshi, M., Miyashita, K., Komatsu, S., Nishimura, N., Shimizu, K., & Hori, Y. (2025). Novel orthotopic patient-derived xenograft model using human pancreatic cancer tissue fragments to recapitulate distant metastasis and cancer-related hypercoagulability. Medical Molecular Morphology: 1–10.
Mutmainnah, M., Chadijah, S., & Rustiah, W. O. (2017). Hidroksiapatit dari Tulang Ikan Tuna Sirip Kuning (Tunnus albacores) dengan Metode Presipitasi. Al-Kimia. 5(2): 119–126.
Najim, M. A., Khalil, B. I., & Hameed, A. A. (2022). Characterizing Optimum Electrospinning Conditions for Graft Urethanized Poly(Vinyl Alcohol)(U-PVA). Heliyon. 8(11): 1–8.
Paneer Selvam, S., Ayyappan, S., I Jamir, S., Sellappan, L. K., & Manoharan, S. (2024). Recent advancements of hydroxyapatite and polyethylene glycol (PEG) composites for tissue engineering applications – A comprehensive review. European Polymer Journal. 215.
Purba, R. A. P., Deswardani, F., Anggraini, R. M., Fendriani, Y., & Restianingsih, T. (2024). Ekstraksi dan karakterisasi hidroksiapatit (HAp) dari tulang ikan tenggiri (Scomberomorus commersoni) dengan metode heat treatment. Jurnal Fisika Unand. 13(2): 247–253.
Rajan, S., Marimuthu, K., Ayyanar, C. B., & Hoque, M. E. (2022). Development and in-vitro characterization of HAP blended PVA/PEG bio-membrane. Journal of Materials Research and Technology. 18: 4956–4964.
Ranamanggala, J. A., Laily, D. I., Annisa, Y. N., & Cahyaningrum, S. E. (2020). Potensi Hidroksiapatit dari Tulang Ayam Sebagai Pelapis Implan Gigi. Jurnal Kimia Riset. 5(2): 141.
Roseti L, Parisi V, Petretta M, Cavallo C, Desando G, Bartolotti I, Grigolo B. (2017). Scaffolds for Bone Tissue Engineering: State of the art and new perspectives. Mater Sci Eng C Mater Biol Appl. 1(78):1246-1262.
Salcedo, S. S., Arcos, D., & Vallet-regí, M. (2008). Upgrading calcium phosphate scaffolds for tissue engineering applications. Engineering Materials. 377: 19–42.
Shang, X., Hu, J., Qu, J., Wen, P., Li, J., Li, Q., & Zheng, J. (2024). Allograft to bone-tunnel integration in a canine anterior cruciate ligament reconstruction model: a comparison study of allograft preparation methods. Journal of Orthopaedic Surgery and Research. 19(1): 1–15.
Susanto, R. (2021). Potensi Pembuatan Replika Tulang Berpori Menggunakan Template Ampas Tebu. Chempublish Journal. 5(2): 116–129.
Syafira, R. S., Devi, M. J., Gaffar, S., & Hartati, Y. W. (2023). Immobilization of Biomolecules on Hydroxyapatite and Its Composites in Biosensor Application: A Review. Biointerface Research in Applied Chemistry. 13(5): 1–17.
Syukur, V. M., Rahyuni, D., Ayuningtyas, E., & Triastianti, R. D. (2024). Pemanfaatan Tulang sapi dan Tulang ayam Menjadi Arang Aktif. Jurnal Rekayasa Lingkungan. 24(2): 17–22.
Szterner, P., & Biernat, M. (2022). Effect of reaction time, heating and stirring rate on the morphology of HAp obtained by hydrothermal synthesis. Journal of Thermal Analysis and Calorimetry. 147(23): 13059–13071.
Teixeira, M. A., Amorim, M. T. P., & Felgueiras, H. P. (2020). Poly ( Vinyl Alcohol ) -Based Nanofibrous Electrospun Sca ff olds for Tissue Engineering Applications.
Triyono, J., Hidayat, T., & Masykur, A. (2020). Karakterisasi dan Laju Biodegradasi Material Biokomposit Bovine Hidroksiapatit ( Bha )/ Ampas Kopi / Shellac sebagai Material Pengisi Tulang. 22(2): 111–118.
Uma Maheshwari, S., Samuel, V. K., & Nagiah, N. (2014). Fabrication and evaluation of (PVA/HAp/PCL) bilayer composites as potential scaffolds for bone tissue regeneration application. Ceramics International. 40(6): 8469–8477.
Vijayaraghavan, P., Rathi, M. A., Almaary, K. S., Alkhattaf, F. S., Elbadawi, Y. B., Woong, S., & Ravindran, B. (2022). Preparation and antibacterial application of hydroxyapatite doped Silver nanoparticles derived from chicken bone. Journal of King Saud University - Science. 34(2).
Wadhwa, H., Kandhol, G., Deshpande, U. P., Mahendia, S., & Kumar, S. (2020). Thermal stability and dielectric relaxation behavior of in situ prepared poly(vinyl alcohol) (PVA)-reduced graphene oxide (RGO) composites. Colloid and Polymer Science. 298(10): 1319–1333.
Wibisono, Y., Ummah, S. R., Hermanto, M. B., Djoyowasito, G., & Noviyanto, A. (2024). Slow-release hydroxyapatite fertilizer from crab shells waste for sustainable crop production. Results in Engineering. 21.
Yusuf, Y., & Diputra, A. H. (2024). Konsep Dasar Scaffold Biomaterial dan Rekayasa Jaringan. Gadjah Mada University Press.
Yusuf, Y., Khasanah, D. U., Syafaat, F. Y., Pawarangan, I., Sari, M., Mawuntu, V. J., & Rizkayanti, Y. (2019). Hidroksiapatit Berbahan Dasar Biogenik (1st ed.). Gadjah Mada University Press.
Yusuf, Y., Khasanah, D. U., Syafaat, F. Y., Pawarangan, I., Sari, M., Mawuntu, V. J., & Rizkayanti, Y. (2019b). Hidroksiapatit Berbahan Dasar Biogenik (Ifan (ed.)). Gadjah Mada University Press.
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Copyright (c) 2025 Meisa Rohania, Lamria Br Galingging, Rahmah Dhani Fitri, Aulia Putri Berliana, Ria Anjelina, Rista Mutia Anggraini
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