Antioxidant Activity of Rhizophora apiculata Methanol Leaves Extract

Authors

  • Husnul Khatimah Ramli Fisheries Resources Utilization Study Program, Faculty of Fisheries and Marine Science, Khairun University
  • Eka Aprianti Fisheries Resources Utilization Study Program, Faculty of Fisheries and Marine Science, Khairun University
  • Riris Roiska Fisheries Resources Utilization Study Program, Faculty of Animal Husbandry, Universitas Jambi
  • M. Aksa Azis Fisheries Resources Utilization Study Program, Faculty of Fisheries and Marine Science, Khairun University
  • Putinur Putinur Fisheries Resources Utilization Study Program, Faculty of Animal Husbandry, Universitas Jambi

DOI:

https://doi.org/10.22437/mjf.v2i02.43770

Keywords:

bioactive compound, DPPH, mangrove

Abstract

Mangroves are well-known for their diverse benefits, with all plant parts containing beneficial bioactive compounds. This study aims to evaluate the antioxidant activity of the methanolic extract of Rhizophora apiculata mangrove leaves. Mature mangrove leaves were extracted using 70% methanol through a maceration method, and antioxidant activity was assessed using the DPPH radical scavenging assay. The results indicated that the extract exhibited inhibition percentages ranging from 56% to 91% at concentrations of 50–1000 ppm, with an IC₅₀ value of 30.86 µg/mL. These findings suggest that the methanolic extract of mature R. apiculata leaves possesses powerful antioxidant potential (IC₅₀ < 50 µg/mL).

 

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References

Akasia, A. I., Nurweda Putra, I. D. N., & Giri Putra, I. N. (2021). Skrining Fitokimia Ekstrak Daun Mangrove Rhizophora mucronata dan Rhizophora apiculata yang Dikoleksi dari Kawasan Mangrove Desa Tuban, Bali (Phytochemical Screening of Leaf Extracts from Rhizophora mucronata and Rhizophora apiculata Mangroves Collected from the Mangrove Area of Tuban Village, Bali). Journal of Marine Research and Technology, 4(1). https://doi.org/10.24843/jmrt.2021.v04.i01.p03

Arifanti, V. B., Sidik, F., Mulyanto, B., Susilowati, A., Wahyuni, T., Subarno, Yulianti, Yuniarti, N., Aminah, A., Suita, E., Karlina, E., Suharti, S., Pratiwi, Turjaman, M., Hidayat, A., Rachmat, H. H., Imanuddin, R., Yeny, I., Darwiati, W., Novita, N. (2022). Challenges and Strategies for Sustainable Mangrove Management in Indonesia: A Review. In Forests (Vol. 13, Issue 5). https://doi.org/10.3390/f13050695

Arifin, M. H., Jacoeb, A. M., Abdullah, A., & Riyadi, P. H. (2023). Drying method comparison of black mangrove leaves (Rhizophora mucronata) for an antioxidant activity assay. Food Research, 7, 67–75. https://doi.org/10.26656/fr.2017.7(S3).10

Bachtiar, B., Ura, R., & Suhartati. (2023). Karakteristik Tapak Tegakan Hutan Mangrove (Rhizophora mucronata dan Avicennia marina) di Pantai Kelurahan Bira Kecamatan Tamalanrea Kota Makassar (Characteristics of Mangrove Forest Stands (Rhizophora mucronata and Avicennia marina) on the Coast of Bira Village, Tamalanrea District, Makassar City). Jurnal Ilmu Alam Dan Lingkungan, 14(1).

Badan Standarisasi Nasional. (2018). Cara uji antioksidan senyawa bahan alam perairan dengan metode DPPH (2,2-difenil-1-pikrilhidrazil) spektrofotometri (How to test the antioxidant properties of natural aquatic compounds using the DPPH (2,2-diphenyl-1-picrylhydrazil) spectrophotometry method ) (SNI 8623-2018). www.bsn.go.id

C. A’in, S. S., & B. Sulardiono. (2017). Kandungan Gizi Pada Produk Olahan Mangrove (KruMang, BoMang, dan SiMang) Produksi Kelompok Tani “NGUDI MAKARYO.” (Nutritional Content of Mangrove Processed Products (KruMang, BoMang, and SiMang) Produced by the “NGUDI MAKARYO” Farmers Group.) Jurnal INFO, 19(1).

Cadamuro, R. D., da Silveira Bastos, I. M. A., da Silva, I. T., da Cruz, A. C. C., Robl, D., Sandjo, L. P., Alves, S., Lorenzo, J. M., Rodríguez-Lázaro, D., Treichel, H., Steindel, M., & Fongaro, G. (2021). Bioactive compounds from mangrove endophytic fungus and their uses for microorganism control. In Journal of Fungi (Vol. 7, Issue 6). MDPI AG. https://doi.org/10.3390/jof7060455

Dewanto, D. K., Hermawan, R., Muliadin, M., Riyadi, P. H., Aisiah, S., & Tanod, W. A. (2021). PROFIL GC-MS DARI Ekstrak Daun Rhizophora Apiculata Dari Pesisir Teluk Tomini, Sulawesi Tengah Dengan Aktivitas Antibakteri Dan Antioksidan. (Gc-Ms Profile Of Rhizophora Apiculata Leaf Extract From The Tomini Bay Coast, Central Sulawesi, With Antibacterial And Antioxidant Activity.) Jurnal Kelautan: Indonesian Journal of Marine Science and Technology, 14(1). https://doi.org/10.21107/jk.v14i1.8904

Egra, S., Kuspradini, H., Kusuma, I. W., Batubara, I., Imra, Nurjannah, Wahyuni, E., Yamauchi, K., & Mitsunaga, T. (2023). Potential of prospective medicinal plants of Rhizophoraceae from North Kalimantan, Indonesia. Biodiversitas, 24(3). https://doi.org/10.13057/biodiv/d240303

Fadilah, R., Sukainah, A., Taufieq, N. A. S., Putra, R. P., & Adriani, M. (2023). Analysis of Bioactive Compounds Content and Toxicity Test on Mangrove Leaf Extract. IOP Conference Series: Earth and Environmental Science, 1209(1). https://doi.org/10.1088/1755-1315/1209/1/012016

Farhoosh, R., Golmovahhed, G. A., & Khodaparast, M. H. H. (2007). Antioxidant activity of various extracts of old tea leaves and black tea wastes (Camellia sinensis L.). Food Chemistry, 100(1). https://doi.org/10.1016/j.foodchem.2005.09.046

Mahmiah, Sudjarwo, G. W., & Andriyani, F. (2021). Potensi Antioksidan Fraksi Etil Asetat Kulit Batang Bakau Hitam (Rhizophora mucronata (Lamk.)) Dari Pantai Timur Surabaya. (Antioxidant Potential of Ethyl Acetate Fraction from Black Mangrove Bark (Rhizophora mucronata (Lamk.)) from the East Coast of Surabaya.) Jurnal Wiyata, 8(1).

Maleke, Z. F. W., Runtuwene, M. R. J., & Kamu, V. S. (2024). Pengaruh Daun Muda dan Daun Tua Terhadap Aktivitas Antioksidan dan Kualitas Mutu Teh Herbal Daun Soyogik (Saurauia bracteosa DC.). (The Effect of Young Leaves and Old Leaves on Antioxidant Activity and Quality of Soyogik Leaf Herbal Tea (Saurauia bracteosa DC.)) CHEMISTRY PROGRESS, 17(1), 79–86. https://doi.org/10.35799/cp.17.1.2024.49757

Maulana, D. M., & Sasmito, B. B. (2021). The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells. Journal of Stem Cell Research and Tissue Engineering, 5(1). https://doi.org/10.20473/jscrte.v5i1.29380

Mile, L.-, Nursyam, H., Setijawati, D., & Sulistiyati, T. D. (2021). Studi Fitokimia Buah Mangrove (Rhizophora mucronata) Di Desa Langge Kabupaten Gorontalo Utara. (Phytochemical Study of Mangrove Fruit (Rhizophora mucronata) in Langge Village, North Gorontalo Regency.) Jambura Fish Processing Journal, 3(1). https://doi.org/10.37905/jfpj.v3i1.8585

Mohd Mokhtar, R. A., Misrah, M. F., Kansil, T., Amin, Z., Yusof, N. A., & Budiman, C. (2022). Evaluation of antioxidant activity and total phenolics of selected mangrove plants in Sabah. Borneo International Journal of Biotechnology (BIJB), 2. https://doi.org/10.51200/bijb.v2i.3330

Mutik, M. S., Sibero, M. T., Widianingsih, W., Subagiyo, S., Pribadi, R., Haryanti, D., Ambariyanto, A., & Murwani, R. (2022). Kandungan Senyawa Bioaktif dan Aktivitas Biologis Ekstrak Daun Rhizophora apiculata Asal Perairan Teluk Awur, Jepara. (Bioactive Compound Content and Biological Activity of Rhizophora apiculata Leaf Extract from Awur Bay, Jepara.) Jurnal Kelautan Tropis, 25(3). https://doi.org/10.14710/jkt.v25i3.14287

Ramli, H. K., Yuniarti, T., Lita, N. P. S. N., & Sipahutar, Y. H. (2020). Qualitative Phytochemical Test on Mangrove Fruit and Fruit Water Extracts. Journal of Fisheries and Marine Extension, 14(1).

Ridlo, A., Pramesti, R., Supriyantini, E., & Soenardjo, N. (2017). Aktivitas Antioksidan Ekstrak Daun Mangrove Rhizopora mucronata . (Antioxidant Activity of Rhizophora mucronata Mangrove Leaf Extract). Buletin Oseanografi Marina , 6(2), 110–116.

Rumengan, A. P., Mandiangan, E. S., Tanod, W. A., Paransa, D. S. J., Paruntu, C. P., & Mantiri, D. M. H. (2021). Identification of pigment profiles and antioxidant activity of Rhizophora mucronata mangrove leaves from Lembeh, North Sulawesi, Indonesia. Biodiversitas, 22(7). https://doi.org/10.13057/biodiv/d220730

Rusila Noor, Y. , M. Khazali, & I N.N. Suryadiputra. (2006). Panduan Pengenalan Mangrove di Indonesia Part-1. (Guide to Mangroves in Indonesia Part 1.) In Bogor, Ditjen PHKA, Wetlands International, Indonesia Programme cetakan ketiga 2012.

Sari, N. I., Sidauruk, S. W., Dewita, Ananda, N. T., & Leksono, T. (2024). Valorization of mangrove leaves (Sonneratia alba) based on leaf age as a functional salt preparation. Kuwait Journal of Science, 51(1). https://doi.org/10.1016/j.kjs.2023.06.003

Setyawan, A. D., RAGAVAN, P., BASYUNI, M., & SARNO, S. (2022). Review: Rhizophora mucronata as a source of food and medicines. International Journal of Bonorowo Wetlands, 9(1). https://doi.org/10.13057/bonorowo/w090105

Utomo, D. S., Kristiani, E. B. E., & Mahardika, A. (2020). Pengaruh Lokasi Tumbuh Terhadap Kadar Flavonoid, Fenolik, Klorofil, Karotenoid dan Aktivitas Antioksidan Pada Tumbuhan Pecut Kuda (Stachytarpheta Jamaicensis). (The Effect of Growing Location on Flavonoid, Phenolic, Chlorophyll, Carotenoid Content, and Antioxidant Activity in Horsewhip Plant (Stachytarpheta Jamaicensis)). Bioma, 22(2).

Vittaya, L., Charoendat, U., Janyong, S., Ui-eng, J., & Leesakul, N. (2022). Comparative analyses of saponin, phenolic, and flavonoid contents in various parts of Rhizophora mucronata and Rhizophora apiculata and their growth inhibition of aquatic pathogenic bacteria. Journal of Applied Pharmaceutical Science, 12(11). https://doi.org/10.7324/JAPS.2022.121113

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Published

2025-08-26

How to Cite

Ramli, H. K., Aprianti, E., Roiska, R., Azis, M. A., & Putinur, P. (2025). Antioxidant Activity of Rhizophora apiculata Methanol Leaves Extract. Mantis Journal of Fisheries, 2(02), 64–69. https://doi.org/10.22437/mjf.v2i02.43770

Issue

Vol. 2 No. 02 (2025): Agustus 2025

Section

Articles

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Copyright (c) 2025 Husnul Khatimah Ramli, Eka Aprianti, Riris Roiska, M. Aksa Azis, Putinur Putinur

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