Assessing The Impact of Transglutaminase and Methylcellulose on Physical Properties of Seitan-Infused Plant-based Meat Analog Patties Compared to Beef Patties
DOI:
https://doi.org/10.22437/ifstj.v8i2.37001Keywords:
Meat analog, Patties, TVP, Seitan, VolscanAbstract
This study seeks to develop plant-based patties (PBP) by varying TVP-to-seitan ratios (30:70, 50:50, and 70:30) and adjusting methylcellulose (MC) and transglutaminase (TG) concentrations (MC: 2%, TG: 2%, and MC+TG: 1+1%). These formulations were compared to a beef patty (control, CON) across several parameters including appearance, cooking loss, color, texture, and surface area. While PBP exhibited similar appearances, they differed from beef patties in terms of shrinkage. PBP was firmer (0.31-3.18 N) with higher seitan content but less tough than CON (13.77 N). Additionally, PBP showed larger surface areas than CON, and the addition of TG reduced shrinkage and cooking loss, particularly with higher seitan content. Although all PBP appeared darker (lower L* values) than CON, the redness (a*) and yellowness (b*) remained unaffected by the base ingredients and binders. Optimal PBP formulations, containing 70% seitan and either 2% TG or a combination of 1% MC and 1% TG, achieved a balance between texture, reduced shrinkage, and cooking loss but did not perfectly match the color of conventional patties.
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[1] S. Tianyu, L. Bei, Z. Wei , HB. Kathrine, OS. Philip, Z. Zhongquan, "Technological challenges and future perspectives of plant-based meat analogues: From the viewpoint of proteins," Food Research International, vol. 186, p. 114351, 2024.
[2] Ain, B. Fatema Hossain, S. Muhamad, I. Ismail, Norizah, and I.-F. Mohammad Rashedi, "Methylcellulose replacement with different enzymatically treated plant fibres as a binder in the production of plant-based meat patties," Lwt, vol. 201, p. 116231, 2024.
[3] J. H. Han, D. H. Keum, S. J. Hong, Y. J. Kim, and S. G. Han, "Comparative Evaluation of Polysaccharide Binders on the Quality Characteristics of Plant-Based Patties," (in eng), Foods, vol. 12, no. 20, Oct 11 2023.
[4] E. Gupta, V. Singh, and S. Prasad, "Plant Protein and Human Health," in The Future of Plant Protein: Innovations, Challenges, and Opportunities, K. Younis and O. Yousuf, Eds. Singapore: Springer Nature Singapore, 2025, pp. 231-253.
[5] S. Langyan, P. Yadava, F. N. Khan, Z. A. Dar, R. Singh, and A. Kumar, "Sustaining Protein Nutrition Through Plant-Based Foods," (in eng), Front Nutr, vol. 8, p. 772573, 2021.
[6] J. B. Christopher, "Plant-based animal product alternatives are healthier and more environmentally sustainable than animal products," Future Foods, vol. 6, p. 100174, 2022.
[7] I. Aaysha, A. Zia, Z. Jie, B. Muhammad, Hafiz, and H. Aijun, "New trends in functionalities and extraction of plant proteins in designing plant-based meat analogues: A critical review," Food Bioscience, vol. 57, p. 103476, 2024.
[8] Y. Chen, D. Lan, W. Wang, W. Zhang, and Y. Wang, "Effect of transglutaminase-catalyzed crosslinking behavior on the quality characteristics of plant-based burger patties: A comparative study with methylcellulose," (in eng), Food Chem, vol. 428, p. 136754, Dec 1 2023.
[9] Somayeh, Z. Yunyu, V. Bongkosh, and Atze, "Enhancing textural properties in plant-based meat alternatives: The impact of hydrocolloids and salts on soy protein-based products," Current Research in Food Science, vol. 7, p. 100571, 2023.
[10] B. Dekkers, R. Boom, and A. J. Goot, "Structuring processes for meat analogues," Trends in Food Science & Technology, vol. 81, 2018.
[11] K. Sakai, Y. Sato, M. Okada, and S. Yamaguchi, "Improved functional properties of meat analogs by laccase catalyzed protein and pectin crosslinks," Sci Rep, vol. 11, no. 1, p. 16631, Aug 17 2021.
[12] A. Lerner and C. Benzvi, "Microbial transglutaminase is a very frequently used food additive and is a potential inducer of autoimmune/neurodegenerative diseases," (in eng), Toxics, vol. 9, no. 10, p. 233, Sep 25 2021.
[13] M. Kieliszek and A. Misiewicz, "Microbial transglutaminase and its application in the food industry. A review," Folia Microbiol (Praha), vol. 59, no. 3, pp. 241-50, May 2014.
[14] A. Bakhsh, S. J. Lee, E. Y. Lee, N. Sabikun, Y. H. Hwang, and S. T. Joo, "A Novel Approach for Tuning the Physicochemical, Textural, and Sensory Characteristics of Plant-Based Meat Analogs with Different Levels of Methylcellulose Concentration," Foods, vol. 10, no. 3, Mar 8 2021.
[15] A. Bakhsh, S.-J. Lee, E.-Y. Lee, N. Sabikun, Y.-H. Hwang, and S.-T. Joo, "A novel approach for tuning the physicochemical, textural, and sensory characteristics of plant-based meat analogs with different levels of methylcellulose concentration," Foods, vol. 10, no. 3, p. 560, 2021.
[16] W. Ming-Min and M. R. Ismail-Fitry, "Physicochemical, rheological and microstructural properties of chicken meat emulsion with the addition of Chinese yam (Dioscorea polystachya) and arrowroot (Maranta arundinacea) as meat substitutes," Future Foods, vol. 7, p. 100221, 2023/06/01/ 2023.
[17] N. H. Yahya, N. S. Zulkifli, S. N. A. Ramli, I. Ismail, and W. M. F. Wan Mokhtar, "Effects of sous-vide cooking on the initial yield, peak force, and elastic modulus of cooked beef semitendinosus," Journal of Agrobiotechnology, vol. 12, no. 1S, pp. 83-91, 2021.
[18] G. Vu, H. Zhou, and D. J. McClements, "Impact of cooking method on properties of beef and plant-based burgers: Appearance, texture, thermal properties, and shrinkage," Journal of Agriculture and Food Research, vol. 9, 2022.
[19] J. Mann, B. Schiedt, A. Baumann, B. Conde-Petit, and T. A. Vilgis, "Effect of heat treatment on wheat dough rheology and wheat protein solubility," Food Science and Technology International, vol. 20, no. 5, pp. 341-351, 2014.
[20] Y. Liu et al., "Investigating the effects of protein thermal denaturation on the water-holding capacity of beef: insights from structural dynamics," International Journal of Food Science and Technology, vol. 60, no. 1, p. vvaf076, 2025.
[21] A. Bakhsh, S. J. Lee, E. Y. Lee, Y. H. Hwang, and S. T. Joo, "Evaluation of Rheological and Sensory Characteristics of Plant-Based Meat Analog with Comparison to Beef and Pork," (in eng), Food Sci Anim Resour, vol. 41, no. 6, pp. 983-996, Nov 2021.
[22] I. J. Sobieszek and A. Sobieszek, "Myosin assembly of smooth muscle: from ribbons and side polarity to a row polar helical model," Journal of Muscle Research and Cell Motility, vol. 43, no. 3, pp. 113-133, 2022/09/01 2022.
[23] Z. Forghani, M. H. Eskandari, M. Aminlari, and S. S. Shekarforoush, "Effects of microbial transglutaminase on physicochemical properties, electrophoretic patterns and sensory attributes of veggie burger," (in eng), Journal of Food Science and Technology, vol. 54, no. 8, pp. 2203-2213, Jul 2017.
[24] J. Flory, R. Xiao, Y. Li, H. Dogan, M. J. Talavera, and S. Alavi, "Understanding Protein Functionality and Its Impact on Quality of Plant-Based Meat Analogues," Foods, vol. 12, no. 17, p. 3232, 2023.
[25] J. Jang and D. W. Lee, "Advancements in plant based meat analogs enhancing sensory and nutritional attributes," (in eng), NPJ Sci Food, vol. 8, no. 1, p. 50, Aug 7 2024.
[26] I. Ismail, S.-T. Joo, A. Bakhsh, J. Sung-Hyun, and H. Young-Hwa, "The alternative approach of low temperature-long time cooking on bovine semitendinosus meat quality," Asian-Australasian Journal of Animal Sciences, vol. 32, no. 2, pp. 282-289, 2018.
[27] N. N. Ruslan, J. Y. H. Tang, N. Huda, M. R. Ismail-Fitry, and I. Ishamri, "Effects of phosphate and two-stage sous-vide cooking on textural properties of the beef semitendinosus," (in eng), Food Science of Animal Resources, vol. 43, no. 3, pp. 491-501, May 2023.
[28] L. Jiang et al., "Improve the fiber structure and texture properties of plant-based meat analogues by adjusting the ratio of soy protein isolate (SPI) to wheat gluten (WG)," Food Chemistry: X, vol. 24, p. 101962, 2024/12/30/ 2024.
[29] R. Zhang et al., "Effect of Wheat Gluten and Peanut Protein Ratio on the Moisture Distribution and Textural Quality of High-Moisture Extruded Meat Analogs from an Extruder Response Perspective," Foods, vol. 12, no. 8, p. 1696, 2023.
[30] G. Vu, H. Zhou, and D. J. McClements, "Impact of cooking method on properties of beef and plant-based burgers: Appearance, texture, thermal properties, and shrinkage," Journal of Agriculture and Food Research, vol. 9, p. 100355, 2022/09/01/ 2022.
[31] J. Li et al., "Low temperature extrusion promotes transglutaminase cross-linking of whey protein isolate and enhances its emulsifying properties and water holding capacity," Food Hydrocolloids, vol. 125, p. 107410, 2022/04/01/ 2022.
[32] M. L. Coughlin, L. Liberman, S. P. Ertem, J. Edmund, F. S. Bates, and T. P. Lodge, "Methyl cellulose solutions and gels: fibril formation and gelation properties," Progress in Polymer Science, vol. 112, p. 101324, 2021/01/01/ 2021.
[33] K. Kyriakopoulou, J. K. Keppler, and A. J. van der Goot, "Functionality of ingredients and additives in plant-based meat analogues," Foods, vol. 10, no. 3, p. 600, 2021.
[34] Y. Wen, H. W. Kim, and H. J. Park, "Effects of transglutaminase and cooking method on the physicochemical characteristics of 3D-printable meat analogs," Innovative Food Science & Emerging Technologies, vol. 81, p. 103114, 2022/10/01/ 2022.
[35] D. Zhu, Q. Wu, and N. Wang, "3.02 - Industrial Enzymes," in Comprehensive Biotechnology, M. Moo-Young, Ed. 2nd ed. Burlington: Academic Press, 2011, pp. 3-13.
[36] I. Ismail, Y.-H. Hwang, and S.-T. Joo, "Effect of different temperature and time combinations on quality characteristics of sous-vide cooked goat gluteus medius and biceps femoris," Food and Bioprocess Technology, vol. 12, pp. 1000-1009, 2019.
[37] I. Ismail, Y.-H. Hwang, and S.-T. Joo, "Interventions of two-stage thermal sous-vide cooking on the toughness of beef semitendinosus," Meat Science, vol. 157, p. 107882, 2019/11/01/ 2019.
[38] K. Srikaeo, J. Furst, R. Hosken, and J. Ashton, "Physical properties of cooked wheat grains as affected by cooking temperature and duration," International Journal of Food Properties, vol. 8, pp. 469-479, 09/01 2005.
[39] L. Zhang et al., "Effect of wheat gluten on edible quality of crusts from deep-fried battered pork slices," Journal of the Chinese Cereals and Oils Association, vol. 33, pp. 13-18, 09/25 2018.
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