Development and Characterization of New Vegan Ice Cream from Tempeh Milk Enriched with Moringa Leaf Flour
DOI:
https://doi.org/10.22437/ifstj.v8i2.41842Keywords:
calcium, moringa leaf flour, protein, tempeh milk, vegan ice creamAbstract
Abstract— Vegan ice cream is among the currently trending plant-based products, but not all can meet nutritional needs, specifically protein and calcium, which are often found in milk. An effective strategy to address this problem is by adding moringa leaf flour (MLF) and tempeh milk, which are rich in protein and calcium. Therefore, this study aims to develop high-calcium vegan ice cream from tempeh milk enriched with MLF. The experimental method was divided into characterization of MLF and tempeh milk, physical characterization and sensory evaluation of ice cream with Check All That Apply (CATA) method, and chemical characterization for the most preferred recipe. The selected MLF concentrations included 4%, 5%, 6%, and 7%, where ice cream with a higher concentration had harder and stickier texture, as well as a higher melting time, and a smaller overrun value than counterparts developed using less MLF. Sensory evaluation was conducted with 50 untrained panelists, while the must-not-have properties of MLF ice cream were a dark green color, a solid appearance, a bitter taste and aftertaste, greeny flavor, and a hard texture. The most preferred formulation was ice cream containing 4% MLF, with characteristics such as 68.9% water, 0.59% ash, 2.38% protein, 5.43% fat, 22.70% carbohydrate, calcium 93.13 mg/100 g, and iron 1.02 mg/100 g. The best formula did not meet the regulation of calcium and protein source because of the sensory acceptance limitation.
Downloads
References
[1] The State of Food Security and Nutrition in the World 2022. FAO, 2022. doi: 10.4060/cc0639en.
[2] R. Agustina et al., “Nutrient Intake Adequacy among Adults in Indonesia and Malaysia: A Systematic Review and Meta-Analysis,” May 01, 2025, Elsevier B.V. doi: 10.1016/j.cdnut.2025.106010.
[3] S. Sandjaja et al., “Food consumption and nutritional and biochemical status of 0·5-12-year-old Indonesian children: The SEANUTS study,” British Journal of Nutrition, vol. 110, no. SUPPL.3, Sep. 2013, doi: 10.1017/S0007114513002109.
[4] H. R. B. Arini, V. Hadju, P. Thomas, and M. Ferguson, “Nutrient and Food Intake of Indonesian Children Under 5 Years of Age: A Systematic Review,” Asia Pac J Public Health, vol. 34, Oct. 2021, doi: 10.1177/10105395211041001.
[5] P. Sari, D. M. D. Herawati, M. Dhamayanti, and D. Hilmanto, “The Study of Nutrient Intake and Adolescent Girls’ Quality of Life in a Rural Area of Indonesia,” Children, vol. 9, no. 8, Aug. 2022, doi: 10.3390/children9081248.
[6] Improving child nutrition : the achievable imperative for global progress. United Nations Children’s Fund, 2013.
[7] R. L. Peters et al., “The prevalence of food allergy and other allergic diseases in early childhood in a population-based study: HealthNuts age 4-year follow-up,” Journal of Allergy and Clinical Immunology, vol. 140, no. 1, pp. 145-153.e8, Jul. 2017, doi: 10.1016/j.jaci.2017.02.019.
[8] A. Szilagyi and N. Ishayek, “Lactose intolerance, dairy avoidance, and treatment options,” Dec. 15, 2018, MDPI AG. doi: 10.3390/nu10121994.
[9] B. Hegar and A. Widodo, “Lactose intolerance in Indonesian children,” 2015, HEC Press. doi: 10.6133/apjcn.2015.24.s1.06.
[10] Rakuten, “Plant-based food alternatives Future or Present?,” 2021. Accessed: Feb. 14, 2025. [Online]. Available: https://insight.rakuten.com/wordpress/wp-content/uploads/Report_Plant_based_food.pdf
[11] “statistic_id1227682_retail-sales-value-of-ice-cream-and-frozen-desserts-indonesia-2017-2022”.
[12] B. Çabuk et al., “Effect of fermentation on the protein digestibility and levels of non-nutritive compounds of pea protein concentrate,” Food Technol Biotechnol, vol. 56, no. 2, pp. 257–264, Apr. 2018, doi: 10.17113/ftb.56.02.18.5450.
[13] M. Gita Miranti and I. Dianah Wati, “Nutritional Analysis of Non-Dairy Milk Almond-Tempeh as a Multivitamin Supplement for the Elderly,” 2021.
[14] M. Torrens-Mas and P. Roca, “Phytoestrogens for cancer prevention and treatment,” Dec. 01, 2020, MDPI AG. doi: 10.3390/biology9120427.
[15] L. Govender and M. Siwela, “The effect of moringa oleifera leaf powder on the physical quality, nutritional composition and consumer acceptability of white and brown breads,” Foods, vol. 9, no. 12, Dec. 2020, doi: 10.3390/foods9121910.
[16] J. Cao et al., “Moringa oleifera leaf protein: Extraction, characteristics and applications,” Jun. 01, 2023, Academic Press Inc. doi: 10.1016/j.jfca.2023.105234.
[17] R. Wahyuni, W. Wignyanto, S. Wijana, and S. Sucipto, “Optimization of foam mat drying process of moringa leaf powder (Moringa oleifera) as protein and amino acids sources,” Food Res, vol. 5, no. 2, pp. 418–426, Apr. 2021, doi: 10.26656/fr.2017.5(2).539.
[18] I. F. Bolarinwa, T. E. Aruna, and A. O. Raji, “Nutritive value and acceptability of bread fortified with moringa seed powder,” Journal of the Saudi Society of Agricultural Sciences, vol. 18, no. 2, pp. 195–200, Apr. 2019, doi: 10.1016/j.jssas.2017.05.002.
[19] L. Gopalakrishnan, K. Doriya, and D. S. Kumar, “Moringa oleifera: A review on nutritive importance and its medicinal application,” Jun. 01, 2016, Elsevier B.V. doi: 10.1016/j.fshw.2016.04.001.
[20] K. Ahmed et al., “Proximate analysis: Relative feed values of various forage plants for ruminants investigated in a semi-arid region of Punjab, Pakistan,” Agricultural Sciences, vol. 04, no. 06, pp. 302–308, 2013, doi: 10.4236/as.2013.46043.
[21] I. N. Oduro, W. O. Ellis, and D. Owusu, “Nutritional potential of two leafy vegetables: Moringa oleifera and Ipomoea batatas leaves,” Scientific Research and Essays, vol. 3, pp. 57–60, 2008, [Online]. Available: https://api.semanticscholar.org/CorpusID:96853518
[22] M. Saeed, S. W. Ali, and S. Ramzan, “Physicochemical analysis of mango flavored yogurt supplemented with moringa oleifera leaf powder,” J Food Sci Technol, vol. 58, no. 12, pp. 4805–4814, Dec. 2021, doi: 10.1007/s13197-021-05146-w.
[23] Uy. Triastuti and P. Studi Seni Kuliner Akademi Kesejahteraan Sosial Ibu Kartini Semarang, “Making Date Tempe Milk as an Alternative Health Drink,” 2021.
[24] Badan Standardisasi Nasional, “SNI 01-2891-1992 How To Test Foods and Drinks,” 1995.
[25] S. S. Nielsen, Food Analysis Laboratory Manual. in Food Science Text Series. Springer International Publishing, 2017. [Online]. Available: https://books.google.co.id/books?id=xxgnDwAAQBAJ
[26] M. M. M. Makki et al., “Development of a direct observation method and the influence of formulation parameter on frozen ice cream microstructure,” Food Res, vol. 7, no. 2, pp. 186–193, Apr. 2023, doi: 10.26656/fr.2017.7(2).696.
[27] L. A. Lestari, R. A. Wildiana, F. Z. Nisa’, Y. Erwanto, and Y. Pranoto, “Physical, Chemical, and Sensory Properties of Ice Cream with the Substitution of Stabilizer with Gelatin from Various Sources,” Journal of Food and Pharmaceutical Sciences, vol. 7, no. 3, Dec. 2019, doi: 10.22146/jfps.702.
[28] A. Fidyasari, S. Firdauzy, and W. Maslukah, “Physical And Organoleptic Quality Of Tempe Synbiotic Ice Cream With Comparision Of The Mount Of Pineapple Fermentation Result (Ananas Comosus (L) Merr),” Jurnal Inovasi Penelitian, vol. 3, no. 3, Aug. 2022, doi: 10.47492/jip.v3i3.1922.
[29] J. Arcot, S. Wong, and A. K. Shrestha, “Comparison of folate losses in soybean during the preparation of tempeh and soymilk,” J Sci Food Agric, vol. 82, no. 12, pp. 1365–1368, 2002, doi: https://doi.org/10.1002/jsfa.1197.
[30] M. Czarnowska-Kujawska, A. Draszanowska, and M. Starowicz, “Effect of different cooking methods on the folate content, organoleptic and functional properties of broccoli and spinach,” LWT, vol. 167, Sep. 2022, doi: 10.1016/j.lwt.2022.113825.
[31] A. Romulo, B. Meindrawan, and Marpietylie, “Effect of Dairy and Non-Dairy Ingredients on the Physical Characteristic of Ice Cream: Review,” in IOP Conference Series: Earth and Environmental Science, IOP Publishing Ltd, Aug. 2021. doi: 10.1088/1755-1315/794/1/012145.
[32] S. Akram et al., “Nutraceutical properties, biological activities, and industrial applications of chickpea protein,” 2024, Informa Healthcare. doi: 10.1080/23311932.2024.2338653.
[33] F. D. Dakora and A. K. Belane, “Evaluation of Protein and Micronutrient Levels in Edible Cowpea (Vigna Unguiculata L. Walp.) Leaves and Seeds,” Front Sustain Food Syst, vol. 3, 2019, doi: 10.3389/fsufs.2019.00070.
[34] H. Fadhilatunnur, F. Fransisca, and R. T. K. Dewi, “Evaluation Of In Vitro Protein Digestibility Of Moringa Oleifera Leaves With Various Domestic Cooking,” Carpathian Journal of Food Science and Technology, vol. 13, no. 1, pp. 214–222, 2021, doi: 10.34302/crpjfst/2021.13.1.18.
[35] Y. R. Yun et al., “Antioxidant activity and calcium bioaccessibility of Moringa oleifera leaf hydrolysate, as a potential calcium supplement in food,” Food Sci Biotechnol, vol. 29, no. 11, pp. 1563–1571, Nov. 2020, doi: 10.1007/s10068-020-00820-9.
[36] I. C. Antunes, R. Bexiga, C. Pinto, L. C. Roseiro, and M. A. G. Quaresma, “Cow’s Milk in Human Nutrition and the Emergence of Plant-Based Milk Alternatives,” Jan. 01, 2023, MDPI. doi: 10.3390/foods12010099.
[37] A. A. Begum, M. Anisur, and R. Mazumder, “Soymilk as source of nutrient for malnourished population of developing country: A review,” 2016. [Online]. Available: http://www.rspublication.com/ijst/index.html
[38] Y. Durmaz, M. Kilicli, O. S. Toker, N. Konar, I. Palabiyik, and F. Tamtürk, “Using spray-dried microalgae in ice cream formulation as a natural colorant: Effect on physicochemical and functional properties,” Algal Res, vol. 47, p. 101811, May 2020, doi: 10.1016/j.algal.2020.101811.
[39] J. Markowska, A. Tyfa, A. Drabent, and A. Stępniak, “The Physicochemical Properties and Melting Behavior of Ice Cream Fortified with Multimineral Preparation from Red Algae,” Foods, vol. 12, no. 24, Dec. 2023, doi: 10.3390/foods12244481.
[40] A. M. Abdeldaiem, A. H. Ali, A. H. Mousa, W. F. Elkot, and J. Simal-Gandara, “Ice cream supplemented with roasted and grilled corn powders: Physical properties, rheology, antioxidant activity, color, sensory evaluation, and production cost,” Int J Gastron Food Sci, vol. 32, Jun. 2023, doi: 10.1016/j.ijgfs.2023.100692.
[41] E. González-Burgos, I. Ureña-Vacas, M. Sánchez, and M. P. Gómez-Serranillos, “Nutritional value of moringa oleifera Lam. Leaf powder extracts and their neuroprotective effects via antioxidative and mitochondrial regulation,” Nutrients, vol. 13, no. 7, Jul. 2021, doi: 10.3390/nu13072203.
[42] A. Fidyasari, T. Estiasih, S. N. Wulan, and A. Khatib, “The physicochemical, functional, and pasting properties of Moringa oleifera leaf powder from different leaf stalk colors,” CYTA - Journal of Food, vol. 22, no. 1, 2024, doi: 10.1080/19476337.2024.2402062.
[43] H. Bekiroglu, H. Goktas, D. Karaibrahim, F. Bozkurt, and O. Sagdic, “Determination of rheological, melting and sensorial properties and volatile compounds of vegan ice cream produced with fresh and dried walnut milk,” Int J Gastron Food Sci, vol. 28, p. 100521, Jun. 2022, doi: 10.1016/J.IJGFS.2022.100521.
[44] P. Nugroho, L. Hartayanie, and K. P. Dwiana, “The Role of Mungbean (Phaseolus radiatus) as a Fat Replacer on the Physicochemical Properties of Ice Cream,” Indonesian Journal of Agricultural Research, vol. 2, no. 3, pp. 111–120, Jan. 2020, doi: 10.32734/injar.v2i3.2859.
[45] M. C. Milliatti and S. C. da S. Lannes, “Impact of stabilizers on the rheological properties of ice creams,” Food Science and Technology (Brazil), vol. 38, no. 4, pp. 733–739, Oct. 2018, doi: 10.1590/fst.31818.
[46] D. Hami and M. Goli, “Optimization of Vanilla Ice Cream Formula by Replacement of Skim Milk Powder with Quinoa Flour by Response Surface Methodology (RSM),” Iranian Food Science and Technology Research Journal, vol. 17, no. 2, pp. 273–285, 2021, doi: 10.22067/ifstrj.2020.39270.
[47] N. Sourial et al., “Correspondence analysis is a useful tool to uncover the relationships among categorical variables,” J Clin Epidemiol, vol. 63, no. 6, pp. 638–646, Jun. 2010, doi: 10.1016/j.jclinepi.2009.08.008.
[48] M. Meyners, J. C. Castura, and B. T. Carr, “Existing and new approaches for the analysis of CATA data,” Food Qual Prefer, vol. 30, no. 2, pp. 309–319, 2013, doi: https://doi.org/10.1016/j.foodqual.2013.06.010.
[49] M. Gorman, R. Moss, and M. B. McSweeney, “Sensory perception of ice cream and plant-based alternatives evaluated blinded and with ingredient lists,” Food and Humanity, vol. 1, pp. 1267–1273, Dec. 2023, doi: 10.1016/J.FOOHUM.2023.09.028.
[50] N. F. Sadek and M. Z. K. Usman, “Exploration of plant milk potentials in moringa leaf ice cream: Physicochemical and sensory evaluations,” in IOP Conference Series: Earth and Environmental Science, Institute of Physics, 2024. doi: 10.1088/1755-1315/1413/1/012068.
[51] G. Ares, C. Dauber, E. Fernández, A. Giménez, and P. Varela, “Penalty analysis based on CATA questions to identify drivers of liking and directions for product reformulation,” Food Qual Prefer, vol. 32, pp. 65–76, Mar. 2014, doi: 10.1016/j.foodqual.2013.05.014.
[52] Y. Jeong, K. W. Lee, H. Kim, and Y. Kim, “Association of milk and dairy product consumption with the incidence of cardio-cerebrovascular disease incidence in middle-aged and older Korean adults: a 16-year follow-up of the Korean Genome and Epidemiology Study,” Nutr Res Pract, vol. 17, no. 6, p. 1225, 2023, doi: 10.4162/nrp.2023.17.6.1225.
[53] Badan Pengawas Obat dan Makanan (BPOM), “Food And Drug Supervisory Agency Regulation No. 1 Of 2022 Regarding The Supervision Of Claims On Processed Food Labels And Advertising,” 2022.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Indonesian Food Science and Technology Journal

This work is licensed under a Creative Commons Attribution 4.0 International License.






