Investigation of the Extraction Parameters on Acquisition of Natural Colorants from Coleus atropurpureus L. Benth Leaves Using Microwave- and Ultrasonic-Assisted Extraction
DOI:
https://doi.org/10.22437/ifstj.v8i2.42509Keywords:
Coleus atropurpureus, Microwave Assisted Extraction, Ultrasonic Assisted Extraction, Natural Colorants, ExtractionAbstract
Abstract— This study systematically investigates the application of Microwave-Assisted Extraction (MAE) and Ultrasonic-Assisted Extraction (UAE) to extract natural pigments from Coleus atropurpureus L. Benth leaves, using water as a green solvent. These advanced techniques were selected over conventional methods due to their well-documented advantages, including shorter extraction times, higher extraction efficiencies, and reduced environmental impact through lower solvent consumption. Importantly, MAE and UAE are particularly suitable for isolating thermolabile compounds such as plant pigments, as they minimize thermal degradation while enhancing yield. Key extraction parameters (feed-to-solvent ratio (F/S), particle size, extraction time, temperature, microwave power, and ultrasonic frequency) were systematically varied to evaluate their influence on extraction performance. Additionally, an air flow system was integrated into both setups to study the effect of controlled airflow (1 to 4 L/min) on pigment yield. For MAE, optimal conditions included 400 W microwave power, an F/S ratio of 0.01 g/mL, particle size greater than 40 mesh, 40 minutes extraction time, and an airflow rate of 1 L/min. In the case of UAE, maximum yield was achieved at a frequency of 40 kHz, extraction temperature of 60°C, F/S ratio of 0.01 g/mL, 25 minutes extraction time, and an airflow rate of 1 L/min. The resulting extracts were characterized using phytochemical screening, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and gas chromatography (GC). Kinetic modeling, employing first- and second-order equations, demonstrated that the second-order kinetic model provided a better fit for both MAE and UAE processes, indicating complex interactions between solute and solvent. Overall, this study highlights the potential of MAE and UAE as efficient, eco-friendly alternatives for pigment extraction from Coleus leaves.
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[1] E. G. De Mejia, Q. Zhang, K. Penta, A. Eroglu, and M. A. Lila, “The Colors of Health: Chemistry, Bioactivity, and Market Demand for Colorful Foods and Natural Food Sources of Colorants,” Annu. Rev. Food Sci. Technol., vol. 11, no. October, pp. 145–182, 2020, doi: 10.1146/annurev-food-032519-051729.
[2] R. Varghese and S. Ramamoorthy, “Status of food colorants in India: conflicts and prospects,” J. fur Verbraucherschutz und Leb., vol. 18, no. 2, pp. 107–118, 2023, doi: 10.1007/s00003-023-01427-y.
[3] R. R. Maronpot, S.-M. Hayashi, and M. Bastaki, “Synthetic and Natural Food Colorants,” Foods Food Ingredients J. Japan, vol. 225, no. 2, pp. 100–110, 2020.
[4] R. B. Malabadi, K. P. Kolkar, and R. K. Chalannavar, “Plant Natural Pigment Colorants-Health Benefits: Toxicity of Synthetic or Artificial Food Colorants,” Int. J. Innov. Sci. Res. Rev., vol. 04, no. 10, pp. 3418–3429, 2022.
[5] M. F. Mohamad, D. J. Dailin, S. E. Gomaa, M. Nurjayadi, and H. El Enshasy, “Natural colorant for food: Alternative a healthy,” Int. J. Sci. Technol. Res., vol. 8, no. 11, pp. 3161–3166, 2019.
[6] S. Gala, S. Sumarno, and M. Mahfud, “Optimization of microwave-assisted extraction of natural dyes from jackfruit wood (Artocarpus heterophyllus Lamk) by response surface methodology,” Eng. Appl. Sci. Res., vol. 49, no. 1, pp. 29–35, 2022, doi: 10.14456/easr.2022.3.
[7] Y. Yuniati, K. Handarini, and R. Rahmiati, “Studi Pustaka: Ekstraksi Pewarna Alami dari Tanaman di Indonesia,” Biosci. J. Ilm. Biol., vol. 12, no. 1, p. 1099, 2024, doi: 10.33394/bioscientist.v12i1.11339.
[8] Y. L. Henuk, “Medicinal plants used in traditional medicine and their roles - in Indonesia,” J. Plant Pathol. Microbiol., vol. 8, no. 5, 2017, doi: 10.4172/2157-7471-C1-004.
[9] M. H. Abdillah et al., “Teknik Budidaya Iler (Coleus atropurpureus. L. Benth) sebagai Herba untuk Penanganan Gejala (Demam dan Batuk) Penyintas Covid-19,” Jumat Pertan. J. Pengabdi. Masy., vol. 3, no. 2, pp. 69–72, 2022, doi: 10.32764/abdimasper.v3i2.2585.
[10] J. Tandi, A. Widodo, R. Yanuarty, T. W. Handayani, and M. Kanan, “Phytochemicals, Antioxidant, and Antidiabetic Activities of Ethanol Extract of Coleus Atropurpureus Benth. Leaves,” Rasayan J. Chem., vol. 17, no. 2, pp. 487–492, 2024, doi: 10.31788/RJC.2024.1728528.
[11] J. Sudirman, N. Petapahan, and L. Pakam, “Natural Alternative Staining of Miana Leaves ( Coleus scutellarioides ( L ) benth ) as a substitute for Gentian Violet in Gram Staining of Staphylococcus aureus Bacteria Institut Kesehatan Medistra Lubuk Pakam,” J. Medistra Med. J., vol. 1, no. 2, 2024.
[12] L. O. Siahaan, E. R. F. Hutapea, and R. Tambun, “Ekstraksi Pigmen Antosianin dari Kulit Rambutan (Nephelium lappaceum) dengan Pelarut Etanol,” J. Tek. Kim. USU, vol. 3, no. 3, pp. 32–38, 2014.
[13] Y. Yuniati, K. Handarini, and Mahfud, “Pigment Extraction Method for Anthocyanin Natural Resources in Indonesia: A Review,” ASEAN J. Chem. Eng., vol. 24, no. 1, pp. 65–78, 2024, doi: 10.22146/ajche.12097.
[14] S. Atun, “Metode Isolasi dan Identifikasi Struktur Senyawa Metabolit Bahan Alam,” J. Konserv. Cagar Budaya Borobudur, vol. 8, no. 2, pp. 53–61, 2014.
[15] S. Gala, S. Sumarno, and M. Mahfud, “Comparison of microwave and conventional extraction methods for natural dyes in wood waste of mahogany (Swietenia mahagoni),” J. Appl. Eng. Sci., vol. 18, no. 4, pp. 618–623, 2020, doi: 10.5937/jaes0-23695.
[16] Y. Variyana and M. Mahfud, “Kinetics Study Using Solvent-Free Microwave Extraction of Essential Oil from Allium sativum L.,” Key Eng. Mater., vol. 840, pp. 186–192, Apr. 2020, doi: 10.4028/www.scientific.net/KEM.840.186.
[17] Y. Yuniati, M. D. Cahyani, I. Novidayasa, P. Prihatini, and M. Mahfud, “Ekstraksi Zat Warna Alami Dari Kayu Bakau (Rhizophora Mucronata) Dengan Metode Microwave Assisted Extraction,” ALCHEMY J. Penelit. Kim., vol. In publish, 2021.
[18] Y. Yuniati, D. D. Azmi, E. Nurandriea, L. Qadariyah, and Mahfud, “Parametric Study and Characterization of Sappan Wood (Caesalpinia Sappan Linn) Natural Red Colorant Extract with Ultrasonic Assisted Extraction Method,” ASEAN J. Chem. Eng., vol. 23, no. 1, pp. 103–112, 2023, doi: 10.22146/ajche.77249.
[19] M. Li, V. Coneva, K. R. Robbins, D. Clark, D. Chitwood, and M. Frank, “Quantitative dissection of color patterning in the foliar ornamental coleus,” Plant Physiol., vol. 187, no. 3, pp. 1310–1324, 2021, doi: 10.1093/plphys/kiab393.
[20] K. H. Lin, M. Y. Huang, J. H. Weng, and S. C. Kao, “Photosynthetic activity of red and green leaf sectors in coleus blumei plants as sensed by chlorophyll fluorescence,” Photosynthetica, vol. 57, no. 2, pp. 659–667, 2019, doi: 10.32615/ps.2019.051.
[21] I. Sharma, N. Khare, and A. Rai, “Carotenoids: Sources, Bioavailability and Their Role in Human Nutrition,” 2024, doi: 10.5772/intechopen.113012.
[22] U. Suhendar, N. F. Utami, D. Sutanto, and S. M. Nurdayanty, “Pengaruh Berbagai Metode Ekstraksi Pada Penentuan Kadar Flavonoid Ekstrak Etanol Daun Iler (Plectranthus scutellarioides),” Fitofarmaka J. Ilm. Farm., vol. 10, no. 1, pp. 76–83, 2020, doi: 10.33751/jf.v10i1.2069.
[23] S. Gala, S. Sumarno, and M. Mahfud, “Microwave-assisted Extraction of Natural Dyes from Coleus atropurpureus Leaves: The Effect of Solvent,” in MATEC Web of Conferences, 2018, vol. 156, doi: 10.1051/matecconf/201815606011.
[24] I. Triesty and M. Mahfud, “Ekstraksi Minyak Atsiri dari Gaharu (Aquilaria Malaccensis) dengan Menggunakan Metode Microwave Hydrodistillation dan Soxhlet Extraction,” J. Tek. ITS, vol. 6, no. 2, 2017, doi: 10.12962/j23373539.v6i2.24491.
[25] H. S. Kusuma and M. Mahfud, “Kinetic studies on extraction of essential oil from sandalwood (Santalum album) by microwave air-hydrodistillation method,” Alexandria Eng. J., vol. 57, no. 2, pp. 1163–1172, 2018, doi: 10.1016/j.aej.2017.02.007.
[26] D. R. Widoretno, D. Kunhermanti, M. Mahfud, and L. Qadariyah, “Ekstraksi Kayu Nangka (Artocarpus heterophyllus lam) dengan Pelarut Etanol sebagai Pewarna Tekstil Menggunakan Metode Microwave-Assisted Extraction,” J. Tek. ITS, vol. 5, no. 2, 2017, doi: 10.12962/j23373539.v5i2.16761.
[27] H. S. Kusuma and M. Mahfud, “‘Preliminary study: Kinetics of oil extraction from basil (Ocimum basilicum) by microwave-assisted hydrodistillation and solvent-free microwave extraction’ [SAJCE 21 (2016) 49–53] (S1026918516300154) (10.1016/j.sajce.2016.06.001)),” South African J. Chem. Eng., vol. 25, p. 178, 2018, doi: 10.1016/j.sajce.2018.01.001.
[28] S. Suharyanto and D. A. N. Prima, “Penetapan Kadar Flavonoid Total pada Juice Daun Ubi Jalar Ungu (Ipomoea Batatas L.) yang Berpotensi Sebagai Hepatoprotektor dengan Metode Spektrofotometri UV-Vis,” Cendekia J. Pharm., vol. 4, no. 2, pp. 110–119, 2020, doi: 10.31596/cjp.v4i2.89.
[29] N. P. Dewi, “Uji Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Daun Awar-Awar (Ficus septica Burm. f) dengan Metode Spektrofotometer UV-VIS,” Acta Holistica Pharm., vol. 2, no. 1, pp. 16–24, 2020.
[30] N. Wathan, “Kajian Farmakognostik Tumbuhan Sugi-Sugi (Breynia cernua Muel. Arg.) Asal Amuntai Kalimantan Selatan,” vol. 03, no. 02, pp. 43–48, 2016.
[31] Y. Variyana, A. S. Rezki, D. Ermaya, and M. Mahfud, “Ekstraksi Minyak Nabati dari Biji Kemiri (Aleurites Moluccana L. Willd.) dengan Metode Microwave Hydrodiffusion and Gravity (MHG),” J. Chem. Proses Enggineering, vol. 08 No. 1, no. 3, pp. 7–16, 2023.
[32] H. S. Kusuma, A. Altway, and M. Mahfud, “Solvent-free microwave extraction of essential oil from dried patchouli (Pogostemon cablin Benth) leaves,” J. Ind. Eng. Chem., vol. 58, pp. 343–348, 2018, doi: 10.1016/j.jiec.2017.09.047.
[33] S. H. Sumarlan and Susilo, “Ekstraksi Senyawa Antioksidan Dari Buah Strawberry (Fragaria X Ananassa) dengan Menggunakan Metode Microwave Assisted Extraction (Kajian Waktu Ekstraksi dan Rasio Bahan dengan Pelarut),” J. Keteknikan Pertan. Trop. dan Biosist., vol. 6, no. 1, pp. 40–51, 2018.
[34] Y. Yuniati, S. N. Putri, P. R. R. Sambawa, D. S. Bhuana, and M. Mahfud, “Ekstraksi Minyak Atsiri dari Bunga Mawar (Rosa hybrda L.) dengan Metode Solvent Free Microwave Extraction,” Alchemy, vol. 9, no. 2, pp. 43–47, 2021, doi: 10.18860/al.v9i2.11511.
[35] N. A. A. R. Zahari, G. H. Chong, L. C. Abdullah, and B. L. Chua, “Ultrasonic-assisted extraction (UAE) process on thymol concentration from Plectranthus amboinicus leaves: Kinetic modeling and optimization,” Processes, vol. 8, no. 3, 2020, doi: 10.3390/pr8030322.
[36] A. F. Nur, M. Bahar, Y. H. Hadiwiardjo, and Y. Setyaningsih, “Aktivitas Antibakteri Ekstrak Graptophyllum pictum (L.) Griff Menggunakan Variasi Frekuensi Ultrasonik Terhadap Salmonella Typhi,” Semin. Nas. Ris. Kedokt., vol. 5, no. 1, pp. 43–49, 2024.
[37] Y. Yuniati, P. E. Elim, R. Alfanaar, H. S. Kusuma, and M. Mahfud, “Extraction of Anthocyanin Pigment from Hibiscus sabdariffa L . by Ultrasonic-Assisted Extraction,” IOP Conf. 2nd Int. Conf. Adv. Eng. Technol., vol. 1010, no. 1, p. 012032, 2021.
[38] A. Q. Syafa’Atullah, A. Amira, S. Hidayati, and M. Mahfud, “Anthocyanin from butterfly pea flowers (Clitoria ternatea) by ultrasonic-assisted extraction,” AIP Conf. Proc., vol. 2237, 2020, doi: 10.1063/5.0005289.
[39] Z. Diaconeasa et al., “Anthocyanins From Agro-Industrial Food Waste: Geographical Approach and Methods of Recovery—A Review,” Plants, vol. 12, no. 1, p. 74, 2022, doi: 10.3390/plants12010074.
[40] M. Vinatoru, T. J. Mason, and I. Calinescu, “Ultrasonically assisted extraction (UAE) and microwave assisted extraction (MAE) of functional compounds from plant materials,” TrAC - Trends Anal. Chem., vol. 97, pp. 159–178, 2017, doi: 10.1016/j.trac.2017.09.002.
[41] J. O. Chaves et al., “Extraction of Flavonoids From Natural Sources Using Modern Techniques,” Front. Chem., vol. 8, no. September, 2020, doi: 10.3389/fchem.2020.507887.
[42] A. Younas, S. Kiran, S. Abrar, S. Javed, and T. Gulzar, “Ultrasonic-Assisted Extraction And Characterization of Natural Colorants From Plants and Evaluation of Their Therapeutic Properties and Cytotoxicity,” Polish J. Environ. Stud., vol. 31, no. 6, pp. 5945–5954, 2022, doi: 10.15244/pjoes/151862.
[43] C. O. Perera and M. A. J. Alzahrani, “Ultrasound as a pre-treatment for extraction of bioactive compounds and food safety: A review,” Lwt, vol. 142, no. February, p. 111114, 2021, doi: 10.1016/j.lwt.2021.111114.
[44] E. D. M. D. Frinhani and R. C. De Oliveira, “The applicability of natural colorants in papermaking,” Tappi J., vol. 5, no. 7, pp. 3–7, 2006.
[45] T. Aysu, N. A. Abd Rahman, and A. Sanna, “Catalytic pyrolysis of Tetraselmis and Isochrysis microalgae by nickel ceria based catalysts for hydrocarbon production,” Energy, vol. 103, pp. 205–214, 2016, doi: 10.1016/j.energy.2016.02.055.
[46] S. K. Movaliya, “Extraction of essential oil by Microwave-assisted extraction: A review,” Int. J. Adv. Res. Innov. Ideas Educ., vol. 3, no. 2, p. 5006, 2017.
[47] H. S. Kusuma and M. Mahfud, “Kinetic studies on extraction of essential oil from sandalwood (Santalum album) by microwave air-hydrodistillation method,” Alexandria Eng. J., vol. 57, no. 2, pp. 1163–1172, 2018, doi: 10.1016/j.aej.2017.02.007.
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